草业学报
草業學報
초업학보
PRATACULTURAL SCIENCE
2015年
5期
91-99
,共9页
姚佳%刘信宝%崔鑫%李志华
姚佳%劉信寶%崔鑫%李誌華
요가%류신보%최흠%리지화
扁蓿豆%盐胁迫%光合生理%渗透调节
扁蓿豆%鹽脅迫%光閤生理%滲透調節
편숙두%염협박%광합생리%삼투조절
Melilotoides ruthenica%salt-stress%osmosis adjustment%photosynthetic physiology
采用盆栽法研究不同浓度NaCl(0,50,100,150,200,250,300mmol/L)胁迫对扁蓿豆苗期有机渗透调节物质及光合参数的影响。结果表明,相同胁迫天数下,随盐浓度的升高。扁蓿豆可溶性蛋白含量、可溶性糖含量呈降低趋势,脯氨酸含量呈升高趋势。各个观测期和浓度之间存在差异,胁迫第7和14天,300mmol/L浓度下可溶性蛋白含量显著低于对照(P<0.05);胁迫第7,14和21天,50~100mmol/L浓度下,可溶性糖含量明显高于对照,但胁迫第28天,250~300mmol/L浓度处理显著低于对照(P<0.05)。胁迫第7天,150~300mmol/L浓度处理、胁迫第14天,200~300mmol/L浓度处理、胁迫第21天,250~300mmol/L浓度处理扁蓿豆脯氨酸含量均分别显著高于对照及其他处理(P<0.05)。可见,盐胁迫下,扁蓿豆产生的3种渗透调节物质调节方式不同,盐胁迫对扁蓿豆幼苗的抑制程度与盐浓度、胁迫时间呈正比。不同的胁迫浓度和胁迫时间,扁蓿豆3个观测期光合参数变化不尽相同。胁迫第14天,叶片净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)和胞间CO2浓度(Ci)随盐浓度的升高呈先上升后降低的趋势。50~100mmol/L浓度处理Pn、Tr和Gs显著高于对照,但300mmol/L浓度处理Pn和Tr显著低于对照及其他处理(P<0.05)。胁迫第28天,4个光合参数随盐浓度的升高而降低。叶片气孔限制值(Ls)和水分利用效率(WUE)在胁迫14和28d时,变化规律一致,随盐浓度的升高呈上升趋势。
採用盆栽法研究不同濃度NaCl(0,50,100,150,200,250,300mmol/L)脅迫對扁蓿豆苗期有機滲透調節物質及光閤參數的影響。結果錶明,相同脅迫天數下,隨鹽濃度的升高。扁蓿豆可溶性蛋白含量、可溶性糖含量呈降低趨勢,脯氨痠含量呈升高趨勢。各箇觀測期和濃度之間存在差異,脅迫第7和14天,300mmol/L濃度下可溶性蛋白含量顯著低于對照(P<0.05);脅迫第7,14和21天,50~100mmol/L濃度下,可溶性糖含量明顯高于對照,但脅迫第28天,250~300mmol/L濃度處理顯著低于對照(P<0.05)。脅迫第7天,150~300mmol/L濃度處理、脅迫第14天,200~300mmol/L濃度處理、脅迫第21天,250~300mmol/L濃度處理扁蓿豆脯氨痠含量均分彆顯著高于對照及其他處理(P<0.05)。可見,鹽脅迫下,扁蓿豆產生的3種滲透調節物質調節方式不同,鹽脅迫對扁蓿豆幼苗的抑製程度與鹽濃度、脅迫時間呈正比。不同的脅迫濃度和脅迫時間,扁蓿豆3箇觀測期光閤參數變化不儘相同。脅迫第14天,葉片淨光閤速率(Pn)、蒸騰速率(Tr)、氣孔導度(Gs)和胞間CO2濃度(Ci)隨鹽濃度的升高呈先上升後降低的趨勢。50~100mmol/L濃度處理Pn、Tr和Gs顯著高于對照,但300mmol/L濃度處理Pn和Tr顯著低于對照及其他處理(P<0.05)。脅迫第28天,4箇光閤參數隨鹽濃度的升高而降低。葉片氣孔限製值(Ls)和水分利用效率(WUE)在脅迫14和28d時,變化規律一緻,隨鹽濃度的升高呈上升趨勢。
채용분재법연구불동농도NaCl(0,50,100,150,200,250,300mmol/L)협박대편숙두묘기유궤삼투조절물질급광합삼수적영향。결과표명,상동협박천수하,수염농도적승고。편숙두가용성단백함량、가용성당함량정강저추세,포안산함량정승고추세。각개관측기화농도지간존재차이,협박제7화14천,300mmol/L농도하가용성단백함량현저저우대조(P<0.05);협박제7,14화21천,50~100mmol/L농도하,가용성당함량명현고우대조,단협박제28천,250~300mmol/L농도처리현저저우대조(P<0.05)。협박제7천,150~300mmol/L농도처리、협박제14천,200~300mmol/L농도처리、협박제21천,250~300mmol/L농도처리편숙두포안산함량균분별현저고우대조급기타처리(P<0.05)。가견,염협박하,편숙두산생적3충삼투조절물질조절방식불동,염협박대편숙두유묘적억제정도여염농도、협박시간정정비。불동적협박농도화협박시간,편숙두3개관측기광합삼수변화불진상동。협박제14천,협편정광합속솔(Pn)、증등속솔(Tr)、기공도도(Gs)화포간CO2농도(Ci)수염농도적승고정선상승후강저적추세。50~100mmol/L농도처리Pn、Tr화Gs현저고우대조,단300mmol/L농도처리Pn화Tr현저저우대조급기타처리(P<0.05)。협박제28천,4개광합삼수수염농도적승고이강저。협편기공한제치(Ls)화수분이용효솔(WUE)재협박14화28d시,변화규률일치,수염농도적승고정상승추세。
The effects of various levels of NaCl stress [0 (control),50,100,150,200,250,300 mmol/L]on the osmotic adjustment matters and photosynthetic physiology of Melilotoides ruthenica seedlings were investi-gated in pot experiments.Soluble protein and sugar contents of seedlings decreased with increasing NaCl con-centration.However,proline content increased with increased NaCl concentration.The contents of soluble protein,sugars,and proline differed under different salt concentrations and stress exposure times.On the 7 th day and 14 th day after imposing stress,soluble protein contents of seedlings exposed to 300 mmol/L NaCl con-centrations were significantly lower than those of controls (P <0.05).On the 7 th ,14 th and 21 st day after impos-ing stress,soluble sugar contents of seedlings exposed to 50 or 100 mmol/L NaCl concentration were higher than those of the controls,while on 28 th day after stress,soluble sugar contents of seedlings exposed to 250 or 300 mmol/L NaCl concentrations were significantly lower than those of the controls (P <0.05).On the 7 th day after imposing stress (150-300 mmol/L NaCl),the 14 th day after imposing stress (200-300 mmol/L NaCl) and on the 28 th day after imposing stress (250 or 300 mmol/L NaCl),the proline content was significantly higher than that of the control and other treatments (P <0.05).Hence under salt stress,three indicators of osmotic adjustment had different time signatures.With increases in both NaCl concentration and stress expo-sure time,the physical damage to M.ruthenica seedlings became more serious,while photosynthetic parame-ters varied with NaCl concentration and stress exposure time.On the 14 th day after imposing stress,photosyn-thetic rate (Pn ),transpiration rate (Tr ),stomatal conductance (Gs)and intercellular CO2 concentration (Ci ) increased at low,and decreased at high salt concentration.Pn ,Tr ,and Gs of seedlings under 50-100 mmol/L concentrations were significantly higher than those of control plants,while Pn and Tr of seedlings under 300 mmol/L were significantly lower than those of control plants and of other treatments (P <0.05).On 28 th day after imposing stress,Pn ,Tr ,Gs and Ci were decreased with increasing of salt concentrations.On the 14 th and 28 th days after imposing stress,stomatal limitation (L s )and water use efficiency (WUE)increased with in-creasing NaCl concentration.