CAJ | 학술논문

目的：研究HE4与Lewis y抗原在卵巢上皮性肿瘤中的表达、相关性及临床意义。方法：免疫组化法检测卵巢上皮性恶性肿瘤、卵巢上皮性交界性肿瘤、卵巢上皮性良性肿瘤及正常卵巢组织中HE4与Lewis y抗原的表达。应用免疫荧光双标记方法检测卵巢上皮性肿瘤中HE4与Lewis y的结构关系。结果：HE4在卵巢恶性肿瘤组的阳性率最高，明显高于良性及正常卵巢组（ p均﹤0.05）。Lewis y表达与HE4相似，在卵巢恶性上皮性肿瘤中，Lewis y抗原的阳性表达率明显高于交界性、良性及正常卵巢组。相关性分析显示：HE4与Lewis y呈直线相关（ r＝0.813，p﹤0.05）。在激光共聚焦显微镜下可见HE4与Lewis y抗原有空间位置上的重叠。结论：HE4与Lewis y抗原在卵巢恶性肿瘤中均呈现明显高表达，且两者在卵巢上皮性肿瘤中的表达呈正相关。
목적：연구HE4여Lewis y항원재란소상피성종류중적표체、상관성급림상의의。방법：면역조화법검측란소상피성악성종류、란소상피성교계성종류、란소상피성량성종류급정상란소조직중HE4여Lewis y항원적표체。응용면역형광쌍표기방법검측란소상피성종류중HE4여Lewis y적결구관계。결과：HE4재란소악성종류조적양성솔최고，명현고우량성급정상란소조（ p균﹤0.05）。Lewis y표체여HE4상사，재란소악성상피성종류중，Lewis y항원적양성표체솔명현고우교계성、량성급정상란소조。상관성분석현시：HE4여Lewis y정직선상관（ r＝0.813，p﹤0.05）。재격광공취초현미경하가견HE4여Lewis y항원유공간위치상적중첩。결론：HE4여Lewis y항원재란소악성종류중균정현명현고표체，차량자재란소상피성종류중적표체정정상관。
Objective:To investigate the expression and the clinical significance of HE4 and Lewis y in ovarian epithelial carcinoma,then evaluate the association between them. Methods:HE4 and Lewis y antigen expression were detectd on tissues from malignant,borderline,and benign ovarian tumors and normal tissues. Their expression and re-lationship were assessed in paraffin sections using immunohistochemistry method. The structure relationship between HE4 and Lewis y antigen was detected by confocal laser scanning microscopy. Results:The results of the immunohis-tochemistry displayed that positive expression rates of HE4 in malignant and boundary ovarian tissues were significant-ly higher than those in benign tumor,similar to Lewis y antigen levels in ovarian cancer. The positive expression rates in malignant ovarian cancer were markedly higher than those in the borderline,benign tumor and normal tissue groups. The correlation analysis showed that tissues displaying marked expression of HE4 simultaneously express high levels of Lewis y antigen. Overall,a linear correlation(r=0. 813,p﹤0. 05)was observed between HE4 and Lewis y antigen in ovarian cancer. In addition,using the double-labeling immunofluorescence method,we observed that HE4 and Lewis y are located in the same position. Conclusion:A close correlation was observed between HE4 and Lewis y antigen which both displayed a notably high level in ovarian cancer.