CAJ | 학술논문

目的：探讨长链非编码RNA MEG3在临床胃癌组织及细胞中的表达情况，以及过表达MEG3对人胃癌细胞增殖能力的影响。方法：采用实时定量PCR检测人胃癌组织及细胞系中MEG3的表达，并结合病理资料分析胃癌组织MEG3表达与临床病理特征之间的关系。采用四甲基偶氮唑蓝（ MTT）法和克隆形成实验检测过表达MEG3前后胃癌细胞增殖的变化。结果：相对于正常胃组织及细胞，在胃癌组织和细胞中MEG3的表达水平明显下调。胃癌组织中MEG3表达与组织学分级、肿瘤浸润深度和TNM分期相关，但与年龄、性别及区域淋巴结转移无关。在SGC-7901细胞中转染MEG3过表达质粒后能显著上调MEG3的表达水平。过表达MEG3能明显抑制胃癌细胞的增殖能力。结论：MEG3对胃癌细胞增殖的调控至关重要，过表达MEG3能明显抑制胃癌细胞的增殖能力。提示MEG3的表达下调可能在胃癌的发生、发展中起重要作用。
목적：탐토장련비편마RNA MEG3재림상위암조직급세포중적표체정황，이급과표체MEG3대인위암세포증식능력적영향。방법：채용실시정량PCR검측인위암조직급세포계중MEG3적표체，병결합병리자료분석위암조직MEG3표체여림상병리특정지간적관계。채용사갑기우담서람（ MTT）법화극륭형성실험검측과표체MEG3전후위암세포증식적변화。결과：상대우정상위조직급세포，재위암조직화세포중MEG3적표체수평명현하조。위암조직중MEG3표체여조직학분급、종류침윤심도화TNM분기상관，단여년령、성별급구역림파결전이무관。재SGC-7901세포중전염MEG3과표체질립후능현저상조MEG3적표체수평。과표체MEG3능명현억제위암세포적증식능력。결론：MEG3대위암세포증식적조공지관중요，과표체MEG3능명현억제위암세포적증식능력。제시MEG3적표체하조가능재위암적발생、발전중기중요작용。
Objective:To investigate the expression level of MEG3 in gastric cancer tissues and cell lines,and study the effect of overexpression of MEG3 expression on gastric cancer cells proliferation. Methods:Real -time quantitative PCR was performed to detect the relative expression of MEG3 in gastric cancer cell lines and tissues. And we explored the correlation of MEG3 expression level with the clinic-pathological factors in gastric cancer patients. MTT and colony formation assays were conducted to detect the effect of MEG3 on gastric cancer cells proliferation. Results:MEG3 expression was lower both in gastric cancer samples and cell lines compared with their corresponding normal tissues and cell lines. The results demonstrated that the expression of MEG3 was correlated with histological grade,depth of tumor invasion and TNM stage,but not with age,gender and lymph node metastasis. Overexpression of MEG3 could significantly inhibit SGC-7901 cells proliferation. Conclusion:Overexpression of MEG3 can affect the proliferation of gastric cancer cell. It is possible that MEG3 may play a key role in the occurrence and development of gastric cancer.