现代肿瘤医学
現代腫瘤醫學
현대종류의학
JOURNAL OF MODERN ONCOLOGY
2015年
11期
1499-1504
,共6页
胃癌细胞%蝎毒%眼镜蛇毒%凋亡%Bcl-2基因
胃癌細胞%蝎毒%眼鏡蛇毒%凋亡%Bcl-2基因
위암세포%갈독%안경사독%조망%Bcl-2기인
gastric cancer cell%scorpid poison%cobra venom%apoptosis%Bcl-2
目的：探讨蝎毒（ scorpid poison）与眼镜蛇毒（ cobra venom）联合应用对胃癌细胞BGC-803凋亡的影响及作用机制。方法：用蝎毒和（或）眼镜蛇毒处理体外传代培养的胃癌细胞株BGC-803。荧光显微镜下观察细胞增殖变化、进行活细胞计数及计算细胞增殖抑制率，流式细胞仪检测细胞凋亡，RT-PCR观察凋亡相关基因Bcl-2表达的变化。结果：蝎毒与眼镜蛇毒联合应用相对于单用蝎毒或眼镜蛇毒可显著提高胃癌细胞凋亡率、抗凋亡基因Bcl-2表达明显下降（ p﹤0.05）。随着蝎毒和眼镜蛇毒合用浓度的增高，胃癌细胞凋亡率增加、抗凋亡基因Bcl-2表达下降更明显（ p﹤0.05）。同一药物浓度作用24h与48h相比，亦有明显差异（p﹤0.05）。结论：蝎毒与眼镜蛇毒联合应用具有协同抗胃癌细胞的作用，并具有浓度和时间依赖性，其机制可能与增强诱导胃癌细胞凋亡及调控凋亡相关基因Bcl-2的表达有关。
目的：探討蝎毒（ scorpid poison）與眼鏡蛇毒（ cobra venom）聯閤應用對胃癌細胞BGC-803凋亡的影響及作用機製。方法：用蝎毒和（或）眼鏡蛇毒處理體外傳代培養的胃癌細胞株BGC-803。熒光顯微鏡下觀察細胞增殖變化、進行活細胞計數及計算細胞增殖抑製率，流式細胞儀檢測細胞凋亡，RT-PCR觀察凋亡相關基因Bcl-2錶達的變化。結果：蝎毒與眼鏡蛇毒聯閤應用相對于單用蝎毒或眼鏡蛇毒可顯著提高胃癌細胞凋亡率、抗凋亡基因Bcl-2錶達明顯下降（ p﹤0.05）。隨著蝎毒和眼鏡蛇毒閤用濃度的增高，胃癌細胞凋亡率增加、抗凋亡基因Bcl-2錶達下降更明顯（ p﹤0.05）。同一藥物濃度作用24h與48h相比，亦有明顯差異（p﹤0.05）。結論：蝎毒與眼鏡蛇毒聯閤應用具有協同抗胃癌細胞的作用，併具有濃度和時間依賴性，其機製可能與增彊誘導胃癌細胞凋亡及調控凋亡相關基因Bcl-2的錶達有關。
목적：탐토갈독（ scorpid poison）여안경사독（ cobra venom）연합응용대위암세포BGC-803조망적영향급작용궤제。방법：용갈독화（혹）안경사독처리체외전대배양적위암세포주BGC-803。형광현미경하관찰세포증식변화、진행활세포계수급계산세포증식억제솔，류식세포의검측세포조망，RT-PCR관찰조망상관기인Bcl-2표체적변화。결과：갈독여안경사독연합응용상대우단용갈독혹안경사독가현저제고위암세포조망솔、항조망기인Bcl-2표체명현하강（ p﹤0.05）。수착갈독화안경사독합용농도적증고，위암세포조망솔증가、항조망기인Bcl-2표체하강경명현（ p﹤0.05）。동일약물농도작용24h여48h상비，역유명현차이（p﹤0.05）。결론：갈독여안경사독연합응용구유협동항위암세포적작용，병구유농도화시간의뢰성，기궤제가능여증강유도위암세포조망급조공조망상관기인Bcl-2적표체유관。
Objective:To study the anti-tumor effects of scorpid poison combination with cobra venom to BGC-803 of gastric cancer cell in vitro and to explore its possible mechanism. Methods:To treat cultured gastric cancer cell line BGC-803 with scorpid poison and( or)cobra venom. Viable cell was counted by trypan blue stain and cell pro-liferation inhibitive rate was calculated,cell apoptosis rate was determined by flow cytometry. Observed expression of apoptosis-related genes of Bcl-2 by RT-PCR. Results:Scorpid poison combined with cobra venom had a stronger inhibition function on BGC-803 of gastric cancer cell than scorpid poison or cobra venom,the expression of Bcl-2 was down(p﹤0. 05). As the concentrations increased of scorpion venom and cobra venom,difference was more sig-nificant(p﹤0. 05). In the same drug concentration effect,24 hours compared to 48 hours also statistically significant (p﹤0. 05). Conclusion:Scorpid poison combined with cobra venom have the synergistic effects on gastric cancer cell,and the mechanisms may be related to apoptosis-inducing effects and through regulating the expression of the related genes Bcl-2.
