现代肿瘤医学
現代腫瘤醫學
현대종류의학
JOURNAL OF MODERN ONCOLOGY
2015年
11期
1479-1482
,共4页
乳岩宁方%TAM%雌激素%MCF-7乳腺癌%益气养阴方%裸鼠
乳巖寧方%TAM%雌激素%MCF-7乳腺癌%益氣養陰方%裸鼠
유암저방%TAM%자격소%MCF-7유선암%익기양음방%라서
Ruyanning decoction%Tamoxifen%estrogen%MCF-7 mammary cancer%Yiqi Yangyin decoction%nude mice
目的：观察中药复方对荷瘤裸鼠一般状态及血清雌激素水平的影响。方法：将造模成功小鼠随机分为对照组、三苯氧胺（TAM）组、乳结合组（乳岩宁方＋TAM）和益结合组（益气养阴方＋TAM）。每日灌胃一次，连续给药21天，隔日测量裸鼠体重，绘制体重变化曲线；分别于用药前、实验第10天、21天用小鼠自主活动测试仪检测小鼠5分钟内自主活动次数。处死裸鼠前摘眼球取血，ELISA法检测小鼠血清中雌二醇（ E2）和孕酮（Progesitn，简称P）水平。结果：体重：TAM组裸鼠体重下降最明显，为（3.18±0.048）g，乳结合组次之，为（2.5±0.034）g，益结合组（1.3±0.019）g，各组间比较有统计学意义（ p﹤0.01）。自主活动次数：实验第10、21天检测裸鼠活动次数，各实验组明显低于其余各组（p﹤0.01）。雌激素水平：含TAM组血清中雌二醇（E2）和孕酮（P）的水平明显低于对照组（p﹤0.01），乳结合组的E2水平明显低于TAM组（p﹤0.01），益结合组的E2水平与TAM组对比无统计学意义（ p﹥0.05），而两个中药结合组的孕酮水平明显低于对照组（ p
目的：觀察中藥複方對荷瘤裸鼠一般狀態及血清雌激素水平的影響。方法：將造模成功小鼠隨機分為對照組、三苯氧胺（TAM）組、乳結閤組（乳巖寧方＋TAM）和益結閤組（益氣養陰方＋TAM）。每日灌胃一次，連續給藥21天，隔日測量裸鼠體重，繪製體重變化麯線；分彆于用藥前、實驗第10天、21天用小鼠自主活動測試儀檢測小鼠5分鐘內自主活動次數。處死裸鼠前摘眼毬取血，ELISA法檢測小鼠血清中雌二醇（ E2）和孕酮（Progesitn，簡稱P）水平。結果：體重：TAM組裸鼠體重下降最明顯，為（3.18±0.048）g，乳結閤組次之，為（2.5±0.034）g，益結閤組（1.3±0.019）g，各組間比較有統計學意義（ p﹤0.01）。自主活動次數：實驗第10、21天檢測裸鼠活動次數，各實驗組明顯低于其餘各組（p﹤0.01）。雌激素水平：含TAM組血清中雌二醇（E2）和孕酮（P）的水平明顯低于對照組（p﹤0.01），乳結閤組的E2水平明顯低于TAM組（p﹤0.01），益結閤組的E2水平與TAM組對比無統計學意義（ p﹥0.05），而兩箇中藥結閤組的孕酮水平明顯低于對照組（ p
목적：관찰중약복방대하류라서일반상태급혈청자격소수평적영향。방법：장조모성공소서수궤분위대조조、삼분양알（TAM）조、유결합조（유암저방＋TAM）화익결합조（익기양음방＋TAM）。매일관위일차，련속급약21천，격일측량라서체중，회제체중변화곡선；분별우용약전、실험제10천、21천용소서자주활동측시의검측소서5분종내자주활동차수。처사라서전적안구취혈，ELISA법검측소서혈청중자이순（ E2）화잉동（Progesitn，간칭P）수평。결과：체중：TAM조라서체중하강최명현，위（3.18±0.048）g，유결합조차지，위（2.5±0.034）g，익결합조（1.3±0.019）g，각조간비교유통계학의의（ p﹤0.01）。자주활동차수：실험제10、21천검측라서활동차수，각실험조명현저우기여각조（p﹤0.01）。자격소수평：함TAM조혈청중자이순（E2）화잉동（P）적수평명현저우대조조（p﹤0.01），유결합조적E2수평명현저우TAM조（p﹤0.01），익결합조적E2수평여TAM조대비무통계학의의（ p﹥0.05），이량개중약결합조적잉동수평명현저우대조조（ p
Objective:To observe the effect of Chinese Herbal Compound formula on the general state and estrogen of tumor-bearing nude mice. Methods:Mice were randomly divided into control group,tamoxifen( TAM)group,Ru combined group( Ruyanning Recipe+TAM)and( Yiqi Yangyin Recipe +TAM). Then decoction was given to mice for 21 days,body weight was recorded every other day and drew the curved line of weight change,the ability of activity was detected before experiment,on the tenth and twenty-first day. The serum Estradiol(E2)and Progesterone(P) level of mice was tested by ELISA method. Results:Body weight:The decline of body weight in TAM group was the most significant,Ru combined group was second ,Yi combined group was third ,the difference was significant( p﹤0. 01). Automatic activity:TAM group was lower than other groups(p﹤0. 01). Estrogen level:the serum E2 and P level in TAM group was lower than the control group(p﹤0. 01),the serum E2 of Ru combined group was lower than TAM group(p﹤0. 01),but there was no significant difference between Yi combined group and TAM group. The two combined groups could decrease the serum P level when compared with that of the control(p﹤0. 01). Conclusion:Chinese Herbal Compound formula can enhance the general state of mice,decrease the decline of body weight,in-crease the ability of activity,can decrease the level of estrogen obviously on tumor-bearing nude mice.
