CAJ | 학술논문

目的：建立一种简便的鼠尾胶原包被的胃癌细胞体外三维培养和药敏技术，初步评估该方法的稳定性和可行性。方法：以国产鼠尾胶原溶液为原料，制备包裹有胃癌细胞株的胶滴，在培养基中培养8天，观察BGC823细胞株在胶滴中的生长情况。接着对胶滴中的细胞克隆进行染色，捕捉胶滴中的数字图像，利用软件计算图像内染色区域的积分光密度值（ integrated optical density，IOD），对胶滴中的细胞活力进行定量；最后初步计算两种不同浓度的顺铂组BGC823细胞株的存活率。结果：成功建立一种新型的胃癌细胞鼠尾胶滴三维培养模型。结论：初步证实了基于此模型进行后续药敏实验的可行性。
목적：건립일충간편적서미효원포피적위암세포체외삼유배양화약민기술，초보평고해방법적은정성화가행성。방법：이국산서미효원용액위원료，제비포과유위암세포주적효적，재배양기중배양8천，관찰BGC823세포주재효적중적생장정황。접착대효적중적세포극륭진행염색，포착효적중적수자도상，이용연건계산도상내염색구역적적분광밀도치（ integrated optical density，IOD），대효적중적세포활력진행정량；최후초보계산량충불동농도적순박조BGC823세포주적존활솔。결과：성공건립일충신형적위암세포서미효적삼유배양모형。결론：초보증실료기우차모형진행후속약민실험적가행성。
Objective:To establish a novel gastric cancer cell 3D culture model,and further investigate the possi-bility of the chemosensitivity test based on that model. Methods:We choose rat-tail collagen to build a cell-embed-ded droplet and we confirmed that the BGC823 gastric cell lines were grown in the droplet,in an aggregation growth mode for 8 days. The colonies in the droplet were then stained by neutral red staining solution. An inverted microscope with CCD camera was used to acquire high-definition images. We further calculated the integrated optical density ( IOD)of stained area in droplet image,and compared the surviving rate of two group treated by CDDP with different concentrations. Results:we successfully established a novel gastric cancer cell 3D culture model. Conclusion:The pssibility of further chemosensitivity test based on that model was confirmed in preliminary.