中国调味品
中國調味品
중국조미품
CHINESE CONDIMENT
2015年
5期
93-96
,共4页
小茴香%黄酮%槲皮素%山奈酚%高效液相色谱
小茴香%黃酮%槲皮素%山奈酚%高效液相色譜
소회향%황동%곡피소%산내분%고효액상색보
Foeniculum vulgare Mill.%quercetin%kaempferol%RP-HPLC
建立用反相高效液相色谱法同时测定小茴香中槲皮素和山奈酚含量的方法。采用 Eclipse XDB-C18色谱柱(4.6 mm×150 mm,5μm);柱温:25℃;流动相:甲醇-0.4%磷酸(50∶50);流速:1 mL/min;检测波长:360 nm。槲皮素、山奈酚分别在2.19~109.6μg/mL 和2.28~114.0μg/mL 范围内线性关系良好。槲皮素和山奈酚的平均回收率分别为95.61%,94.41%,RSD 分别为2.4%,3.0%。此方法简便、准确、可靠,可作为小茴香中槲皮素和山奈酚的定量分析方法。测定出小茴香总黄酮水解物中槲皮素和山奈酚的含量分别为:槲皮素0.29 mg/g,n=3,RSD=2.60%;山奈酚含量为:0.36 mg/g,n=3,RSD=3.25%。
建立用反相高效液相色譜法同時測定小茴香中槲皮素和山奈酚含量的方法。採用 Eclipse XDB-C18色譜柱(4.6 mm×150 mm,5μm);柱溫:25℃;流動相:甲醇-0.4%燐痠(50∶50);流速:1 mL/min;檢測波長:360 nm。槲皮素、山奈酚分彆在2.19~109.6μg/mL 和2.28~114.0μg/mL 範圍內線性關繫良好。槲皮素和山奈酚的平均迴收率分彆為95.61%,94.41%,RSD 分彆為2.4%,3.0%。此方法簡便、準確、可靠,可作為小茴香中槲皮素和山奈酚的定量分析方法。測定齣小茴香總黃酮水解物中槲皮素和山奈酚的含量分彆為:槲皮素0.29 mg/g,n=3,RSD=2.60%;山奈酚含量為:0.36 mg/g,n=3,RSD=3.25%。
건립용반상고효액상색보법동시측정소회향중곡피소화산내분함량적방법。채용 Eclipse XDB-C18색보주(4.6 mm×150 mm,5μm);주온:25℃;류동상:갑순-0.4%린산(50∶50);류속:1 mL/min;검측파장:360 nm。곡피소、산내분분별재2.19~109.6μg/mL 화2.28~114.0μg/mL 범위내선성관계량호。곡피소화산내분적평균회수솔분별위95.61%,94.41%,RSD 분별위2.4%,3.0%。차방법간편、준학、가고,가작위소회향중곡피소화산내분적정량분석방법。측정출소회향총황동수해물중곡피소화산내분적함량분별위:곡피소0.29 mg/g,n=3,RSD=2.60%;산내분함량위:0.36 mg/g,n=3,RSD=3.25%。
The RP-HPLC is applied to determine quercetin and kaempferol content in Foeniculum vulgare Mill..The sample solution is separated by using Zorbax Eclipse (4.6 mm × 150 mm,5μm) as stationary phase and a mixture of methanol and 0.4% phosphoric acid solution (50∶50)as mobile phase.The content of quercetin and kaempferol is determined at the wavelength of 360 nm.The linear relationship between values of peak area and content of quercetin and kaempferol is 2 .1 9 ~109.6μg/mL and 2.28~114.0μg/mL respectively.The recoveries of quercetin and kaempferol are 95.61%,94.41%,with the RSDs of 2.4% and 3.0% respectively.The content of quercetin and kaempferol in flavonoids hydrolysate from Foeniculum vulgare Mill.is 0.29 mg/g (n=3,RSD=2.60%)and 0.36 mg/g (n=3,RSD=3.25%).