创伤外科杂志
創傷外科雜誌
창상외과잡지
JOURNAL OF AUMATIC SURGERY
2015年
3期
210-213
,共4页
刘华%王文明%杨鉴%任光辉
劉華%王文明%楊鑒%任光輝
류화%왕문명%양감%임광휘
颅脑创伤%核糖核酸%预后
顱腦創傷%覈糖覈痠%預後
로뇌창상%핵당핵산%예후
traumatic brain injury%ribanucleic acid%prognosis
目的：探讨miRNA-21在创伤后皮层脑组织中的表达及其与颅脑创伤患者预后的关系。方法分析江苏大学附属昆山医院2011年12月～2013年12月期间行手术治疗的122例颅脑创伤患者的临床资料，男性99例，女性23例；年龄17～84岁，平均（42.19±14.33）岁。采用实时定量逆转录聚合酶链反应检测上述患者创伤后颞叶皮层脑组织标本中miRNA-21的水平。依据miRNA-21的相对表达量将患者分为两组。应用χ2检验分析miRNA-21表达水平与各临床参数的关系；采用Kaplan-Meier法分析miRNA-21表达与颅脑创伤患者生存期之间的关系；采用Cox风险比例模型分析各个协变量的联合效应。结果 miRNA-21的表达量与患者预后相关（χ2＝4.006，P＝0.045），与患者年龄（χ2＝2.941，P＝0.230）、性别（χ2＝2.625，P＝0.105）、入院时格拉斯哥昏迷评分（GCS）（χ2＝1.425，P＝0.490）、致伤原因（χ2＝2.940，P＝0.816）及颅脑创伤类型（χ2＝0.679，P＝0.712）无关；生存分析显示miRNA-21高表达组患者生存期显著高于低表达组（χ2＝9.723，P＝0.002）。 Cox风险比例模型分析显示miRNA-21表达可作为颅脑创伤患者预后的标志。结论 miRNA-21的表达增高提示颅脑创伤患者预后较好，为预测患者病情转归提供一定依据。
目的：探討miRNA-21在創傷後皮層腦組織中的錶達及其與顱腦創傷患者預後的關繫。方法分析江囌大學附屬昆山醫院2011年12月～2013年12月期間行手術治療的122例顱腦創傷患者的臨床資料，男性99例，女性23例；年齡17～84歲，平均（42.19±14.33）歲。採用實時定量逆轉錄聚閤酶鏈反應檢測上述患者創傷後顳葉皮層腦組織標本中miRNA-21的水平。依據miRNA-21的相對錶達量將患者分為兩組。應用χ2檢驗分析miRNA-21錶達水平與各臨床參數的關繫；採用Kaplan-Meier法分析miRNA-21錶達與顱腦創傷患者生存期之間的關繫；採用Cox風險比例模型分析各箇協變量的聯閤效應。結果 miRNA-21的錶達量與患者預後相關（χ2＝4.006，P＝0.045），與患者年齡（χ2＝2.941，P＝0.230）、性彆（χ2＝2.625，P＝0.105）、入院時格拉斯哥昏迷評分（GCS）（χ2＝1.425，P＝0.490）、緻傷原因（χ2＝2.940，P＝0.816）及顱腦創傷類型（χ2＝0.679，P＝0.712）無關；生存分析顯示miRNA-21高錶達組患者生存期顯著高于低錶達組（χ2＝9.723，P＝0.002）。 Cox風險比例模型分析顯示miRNA-21錶達可作為顱腦創傷患者預後的標誌。結論 miRNA-21的錶達增高提示顱腦創傷患者預後較好，為預測患者病情轉歸提供一定依據。
목적：탐토miRNA-21재창상후피층뇌조직중적표체급기여로뇌창상환자예후적관계。방법분석강소대학부속곤산의원2011년12월～2013년12월기간행수술치료적122례로뇌창상환자적림상자료，남성99례，녀성23례；년령17～84세，평균（42.19±14.33）세。채용실시정량역전록취합매련반응검측상술환자창상후섭협피층뇌조직표본중miRNA-21적수평。의거miRNA-21적상대표체량장환자분위량조。응용χ2검험분석miRNA-21표체수평여각림상삼수적관계；채용Kaplan-Meier법분석miRNA-21표체여로뇌창상환자생존기지간적관계；채용Cox풍험비례모형분석각개협변량적연합효응。결과 miRNA-21적표체량여환자예후상관（χ2＝4.006，P＝0.045），여환자년령（χ2＝2.941，P＝0.230）、성별（χ2＝2.625，P＝0.105）、입원시격랍사가혼미평분（GCS）（χ2＝1.425，P＝0.490）、치상원인（χ2＝2.940，P＝0.816）급로뇌창상류형（χ2＝0.679，P＝0.712）무관；생존분석현시miRNA-21고표체조환자생존기현저고우저표체조（χ2＝9.723，P＝0.002）。 Cox풍험비례모형분석현시miRNA-21표체가작위로뇌창상환자예후적표지。결론 miRNA-21적표체증고제시로뇌창상환자예후교호，위예측환자병정전귀제공일정의거。
Objective To explore the expression of miRNA-21 in traumatic brain injury tissues and its cor-relation with prognosis.Methods The clinical characteristics of 122 patients[99 were male and 23 were female with an average age of (42.19 ±14.33) years(17-84 years)] who underwent surgery in our hospital from Dec. 2011 to Dec.2013 were retrospectively analyzed.Real-time PCR was used to detect the expression of miRNA-21.The patients were divided into two groups according to the relative expression of miRNA-21.Chi-square test was uti-lized to analyze the relationship between miRNA-21 expression and clinical features.Survival curves were plotted by using the Kaplan-Meier method and compared by the Log-rank test.A Cox proportional hazard regression model was applied to examine the joint effect of covariants.Results The expression of miRNA-21 was associated with progno-sis (χ2 =4.006,P=0.045).No statistical significance was observed between the expression of miRNA-21 and the clinical parameters,including age(χ2 =2.941,P =0.230),gender(χ2 =2.625,P =0.105),GCS at admission (χ2 =1.425,P=0.490),injury causes(χ2 =2.940,P=0.816) and traumatic brain injury type(χ2 =0.679,P=0.712 ) .Kaplan-Meier analysis showed that high expression of miRNA-21 was closely associated with a longer sur-vival time (χ2 =9.723,P=0.002).Both univariate and multivariate analyses demonstrated that miRNA-21 ex-pression might be a good prognostic marker of traumatic brain injury patients.Conclusion Our results indicate that high-regulation of miRNA-21 is associated with good prognosis of patients with traumatic brain injury and may provide some evidences for the prediction of the prognosis.
