内江师范学院学报
內江師範學院學報
내강사범학원학보
JOURNAL OF NEIJIANG TEACHERS COLLEGE
2015年
4期
42-46,65
,共6页
张婧%莫西%李楠楠%陈围%杨承忠
張婧%莫西%李楠楠%陳圍%楊承忠
장청%막서%리남남%진위%양승충
徐家汇尾孢虫%形态学%分子系统学
徐傢彙尾孢蟲%形態學%分子繫統學
서가회미포충%형태학%분자계통학
Henneguya zikawiensis%morphology%molecular phylogeny
对采集自江西鄱阳湖水域的徐家汇尾孢虫 Henneguya zikawiensis Sikama 1938进行了形态学和分子系统学的研究.徐家汇尾孢虫孢子壳面观呈倒卵形,缝面观呈纺锤形.孢子长11.40μm ±0.94μm(10.0~14.0)μm,宽8.46μm±0.45μm(8.0~10.0)μm,厚7.3μm±0.64μm(7.0~8.0)μm.两个梨形极囊,长4.68μm ±0.43μm(4.0~5.5)μm,3.30μm±0.34μm(2.5~4.0)μm,极丝4~5圈缠绕.基于18S rDNA 的系统发育树、遗传距离、变异位点分析表明,本研究所得到的徐家汇尾孢虫(登陆号为:KR020026)与 GenBank 库中的尾孢虫未定种 Henneguya sp.WB-LZH(登陆号为:JQ690355)是同一物种.另外,系统发育分析并不能把尾孢虫属与碘泡虫属的物种分开,结合形态学比较,进一步证明了尾突不能作为一个有效的分类依据.
對採集自江西鄱暘湖水域的徐傢彙尾孢蟲 Henneguya zikawiensis Sikama 1938進行瞭形態學和分子繫統學的研究.徐傢彙尾孢蟲孢子殼麵觀呈倒卵形,縫麵觀呈紡錘形.孢子長11.40μm ±0.94μm(10.0~14.0)μm,寬8.46μm±0.45μm(8.0~10.0)μm,厚7.3μm±0.64μm(7.0~8.0)μm.兩箇梨形極囊,長4.68μm ±0.43μm(4.0~5.5)μm,3.30μm±0.34μm(2.5~4.0)μm,極絲4~5圈纏繞.基于18S rDNA 的繫統髮育樹、遺傳距離、變異位點分析錶明,本研究所得到的徐傢彙尾孢蟲(登陸號為:KR020026)與 GenBank 庫中的尾孢蟲未定種 Henneguya sp.WB-LZH(登陸號為:JQ690355)是同一物種.另外,繫統髮育分析併不能把尾孢蟲屬與碘泡蟲屬的物種分開,結閤形態學比較,進一步證明瞭尾突不能作為一箇有效的分類依據.
대채집자강서파양호수역적서가회미포충 Henneguya zikawiensis Sikama 1938진행료형태학화분자계통학적연구.서가회미포충포자각면관정도란형,봉면관정방추형.포자장11.40μm ±0.94μm(10.0~14.0)μm,관8.46μm±0.45μm(8.0~10.0)μm,후7.3μm±0.64μm(7.0~8.0)μm.량개리형겁낭,장4.68μm ±0.43μm(4.0~5.5)μm,3.30μm±0.34μm(2.5~4.0)μm,겁사4~5권전요.기우18S rDNA 적계통발육수、유전거리、변이위점분석표명,본연구소득도적서가회미포충(등륙호위:KR020026)여 GenBank 고중적미포충미정충 Henneguya sp.WB-LZH(등륙호위:JQ690355)시동일물충.령외,계통발육분석병불능파미포충속여전포충속적물충분개,결합형태학비교,진일보증명료미돌불능작위일개유효적분류의거.
Henneguya zikawiensis Sikama 1 938,collected from Poyang Lake,Jiangxi province,were used to conduct a morphological and molecular study.It was found that the valve view takes the shape of an obovate and the frontal view a spin-dle.Its spores are 1 1.4 μm ± 0.94 μm(10.0 ~ 14.0)μm long,and 8.46 μm± 0.45 μm(8.0 ~10.0)μm wide and 7.3 μm ± 0.64 μm(7.0 ~ 8.0)μm thick,and each has two polar capsules 4.68 μm± 0.43 μm(4.0 ~ 5.5)μm in length and 3.30μm± 0.34 μm(2.5 ~ 4.0)μm in width with the polar filaments coiling 4 or 5 turns.Analyses of molecular phylogeny,genet-ic distance,variable site based on 18S rDNA indicated that the species (GenBank accession number:KR020026)in the present study is the same species toHenneguya sp.WB-LZH (GenBank accession number:JQ690355 )which has no specific name in the GenBank data base.Additionally,the species of Henneguya can not be separated from species of Myxobolus in the phylo-genetic analysis.This phenomenon combining the morphological comparison could further prove that urogomphus is not an ef-fective proof for the classification of myxosporeans.