CAJ | 학술논문

目的：近年来酒精性脂肪肝发病率逐年增加。模拟人类酒精性脂肪肝发病的病理生理过程，建立酒精性脂肪肝动物模型，通过二维超声监测脂肪肝的动态发展过程，并与病理对照，为深入研究肝纤维化发病机制提供实验模型。方法选取同一批次雄性日本大耳白兔40只，体重1．00～1．52 kg，兔龄（17．01±1．54）d。随机数字表法分为实验组和对照组，每组20只，实验组50°乙醇强行灌胃，2次／d，每次10 mL，常规饲料喂养，自由饮水。对照组常规饲料喂养，自由饮水。2组兔分别于喂养前，喂养12、16、20周共4次动态观察兔脂肪肝的超声表现，处死动物后取肝进行病理观察。结果乙醇喂养12周后18只兔均发生了脂肪肝改变，随着喂饲期的增加，脂肪肝逐渐加重。16周及20周时实验组兔体重明显低于对照组[（2．48±0．30）kg vs （2．78±0．15）kg，（2．61±0．44）kg vs （3．10±0．13）kg， P＜0．05]。超声与病理分级对比，对照组超声与病理结果显示发生脂肪肝0只，实验组发生轻度脂肪肝1只，中度脂肪肝3只，重度脂肪肝13只，而病理结果显示轻度脂肪肝1只，中度脂肪肝4只，重度脂肪肝12只，病理结果与二维超声对脂肪肝的分级基本一致，且20周时病理观察发现纤维化分期以S3-S4期表现为主。结论通过乙醇灌胃可成功制备酒精性脂肪肝的兔模型，超声可作为监测其形成发展的良好手段。
목적：근년래주정성지방간발병솔축년증가。모의인류주정성지방간발병적병리생리과정，건립주정성지방간동물모형，통과이유초성감측지방간적동태발전과정，병여병리대조，위심입연구간섬유화발병궤제제공실험모형。방법선취동일비차웅성일본대이백토40지，체중1．00～1．52 kg，토령（17．01±1．54）d。수궤수자표법분위실험조화대조조，매조20지，실험조50°을순강행관위，2차／d，매차10 mL，상규사료위양，자유음수。대조조상규사료위양，자유음수。2조토분별우위양전，위양12、16、20주공4차동태관찰토지방간적초성표현，처사동물후취간진행병리관찰。결과을순위양12주후18지토균발생료지방간개변，수착위사기적증가，지방간축점가중。16주급20주시실험조토체중명현저우대조조[（2．48±0．30）kg vs （2．78±0．15）kg，（2．61±0．44）kg vs （3．10±0．13）kg， P＜0．05]。초성여병리분급대비，대조조초성여병리결과현시발생지방간0지，실험조발생경도지방간1지，중도지방간3지，중도지방간13지，이병리결과현시경도지방간1지，중도지방간4지，중도지방간12지，병리결과여이유초성대지방간적분급기본일치，차20주시병리관찰발현섬유화분기이S3-S4기표현위주。결론통과을순관위가성공제비주정성지방간적토모형，초성가작위감측기형성발전적량호수단。
Objective The incidence of alcoholic fatty liver increases year by year in recent years .The aim of this study was to establish an animal model of AFL to investigate the pathogenesis of hepatic fibrosis . Methods This study involved 40 male Japa-nese rabbits aged (17.01 ±1.54) d and weighing 1.00-1.52 kg, which were equally randomized to an experimental group and a control group.The animals in the former group received lavage of 10 mL of 50%ethanol twice a day, with normal feedstuff and water, while those in the control group received normal feedstuff and water only .We performed ultrasonography for dynamic liver presentation before and at 12, 16, and 20 weeks after feeding, followed by pathological observation of the livers . Results After 12 weeks of eth-anol garage , fatty liver was observed in 18 of the rabbits and it deteriorated with the prolonged time of administration . The body weight was significantly decreased in the experimental rabbits as com-pared with the controls at 16 weeks ([2.48 ±0.30] vs [2.78 ± 0.15] kg, P<0.05) and 20 weeks ([2.61 ±0.44] vs [3.10 ± 0.13] kg, P<0.05).Ultrasound and pathological grading showed 1 mild, 3 moderate, and 13 severe cases of fatty liver in the experimen-tal group, but none in the control , and pathological examination re- <br> vealed similar results (1 mild, 4 moderate, and 12 severe cases of fatty liver) in the former group.At 20 weeks, alcoholic fatty liver was found mainly in the S3-S4 stage. Conclusion Alcoholic fatty liver models could be successfully established in rabbits by etha-nol garage and ultrasonography is useful for monitoring the development and progression of the condition .