CAJ | 학술논문

目的：观察电针“百会”、“涌泉”两穴对5月龄和10月龄淀粉样前体蛋白/早老蛋白1(amyloid precursor protein/presenilin-1, APP/PS1)双转基因鼠海马β淀粉样蛋白(AmyloidβProtein, Aβ)沉积及超微结构的影响。方法分别将4月龄和9月龄的APP/PS1双转基因小鼠按随机分配法分为模型组、电针组和药物组,以相同月龄同窝阴性小鼠作为对照组,4月龄每组12只,9月龄每组11只。电针组取“百会”、“涌泉”两穴,每次留针时间为15分钟,隔日1次,治疗5周；药物组予多奈哌嗪片0．92 mL/g灌胃,取脑透射电镜片并观察。结果5月龄透射电镜结果显示：在微血管、神经元突触及神经元超微结构上对照组优于模型组。10月龄透射电镜结果显示：模型组海马出现Aβ淀粉样沉淀———老年斑。结论5月龄小鼠行为学出现阿尔茨海默病样改变；10月龄APP/PS1双转基因小鼠海马出现老年斑；电针治疗APP/PS1双转基因小鼠可能对海马超微结构有保护作用。
목적：관찰전침“백회”、“용천”량혈대5월령화10월령정분양전체단백/조로단백1(amyloid precursor protein/presenilin-1, APP/PS1)쌍전기인서해마β정분양단백(AmyloidβProtein, Aβ)침적급초미결구적영향。방법분별장4월령화9월령적APP/PS1쌍전기인소서안수궤분배법분위모형조、전침조화약물조,이상동월령동와음성소서작위대조조,4월령매조12지,9월령매조11지。전침조취“백회”、“용천”량혈,매차류침시간위15분종,격일1차,치료5주；약물조여다내고진편0．92 mL/g관위,취뇌투사전경편병관찰。결과5월령투사전경결과현시：재미혈관、신경원돌촉급신경원초미결구상대조조우우모형조。10월령투사전경결과현시：모형조해마출현Aβ정분양침정———노년반。결론5월령소서행위학출현아이자해묵병양개변；10월령APP/PS1쌍전기인소서해마출현노년반；전침치료APP/PS1쌍전기인소서가능대해마초미결구유보호작용。
Objective To observe the influence of electro-acupuncture(EA) at“Baihui”(GV20) and “Yongquan”(KI1) on hippocampal Aβstain and ultrastructure in 5-month-old and 9-month-old APP/PS1 double transgenic rat. Methods 4-moth-old and 9-month-old APP/PS1 double transgenic rats were according to the random assignment method divided into model group, EA group and drug( AChE) group and drug group with 12 rats each in 4-month-old groups and 11 rats each in 9-month-old groups, and the same months old rats with brood recessive gene were set as control group. Treatment was applied to“Baihui”(GV20) and “Yongquan”(KI1) for 15 mins, once every 2 days for 5weeks;For the drug group, 0. 92 mL/g of acetylcholine enzyme was given by gavage, once a day. Rat brains were taken to make trans-mission electric lens for observation. Results On transmission electron microscopy ( sem) results showed that in 5-month-old rats, the capillaries, synapses and ultrastructure of neurons in the control group were better than the model group. In 10-month-old rats, there was senile plaque in the model group. Conclusion Alzheimer's disease-like behavior appeared in 5-month-old rats. Senile plague in hippocampus appeared in 10-month-old APP/PS1 double transgenic rats. EA therapy might improve the hippocampus microstructure in APP/PS1 double transgenic rats.