CAJ | 학술논문

目的：观察电针委中穴对腰部多裂肌损伤后的干预作用及对胰岛素样生长因子1(insulin like growth factor 1,IGF-1)表达的影响,探求委中穴对大鼠腰肌损伤后再生修复的可能机制。方法雄性SD大鼠120只,随机分为空白组、模型对照组、模型组、电针委中组、电针肾俞组,共5组,观察3个时间点(4天、7天、14天)的变化,每个时间点8个样本。将0.5%布比卡因盐酸盐溶液按每点100μL注射于模型组和电针组大鼠L4、L5水平的多裂肌上。模型对照组采用同样方法注射生理盐水,空白组不做处理。造模后进行电针双侧委中穴或肾俞穴分别治疗4天、7天、14天, HE染色观察肌细胞形态学的改变,免疫组化方法检测肌细胞IGF-1的表达。结果造模前后多裂肌形态学改变显著,损伤后第14天仍未完全恢复。电针委中组与电针肾俞组从形态学上优于模型组。第4、7天模型组多裂肌IGF-1的表达显著高于空白组( P<0．01)；第4天电针委中组表达显著高于模型组(P<0．01),电针委中组表达高于电针肾俞组(P<0．05),电针肾俞组表达高于模型组(P<0．05),而在第14天,电针肾俞组表达显著高于模型组与电针委中组(P<0．01)。结论电针委中穴和电针肾俞穴均能够促进大鼠腰多裂肌损伤后的再生,电针委中穴在肌肉损伤的早期效果显著。
목적：관찰전침위중혈대요부다렬기손상후적간예작용급대이도소양생장인자1(insulin like growth factor 1,IGF-1)표체적영향,탐구위중혈대대서요기손상후재생수복적가능궤제。방법웅성SD대서120지,수궤분위공백조、모형대조조、모형조、전침위중조、전침신유조,공5조,관찰3개시간점(4천、7천、14천)적변화,매개시간점8개양본。장0.5%포비잡인염산염용액안매점100μL주사우모형조화전침조대서L4、L5수평적다렬기상。모형대조조채용동양방법주사생리염수,공백조불주처리。조모후진행전침쌍측위중혈혹신유혈분별치료4천、7천、14천, HE염색관찰기세포형태학적개변,면역조화방법검측기세포IGF-1적표체。결과조모전후다렬기형태학개변현저,손상후제14천잉미완전회복。전침위중조여전침신유조종형태학상우우모형조。제4、7천모형조다렬기IGF-1적표체현저고우공백조( P<0．01)；제4천전침위중조표체현저고우모형조(P<0．01),전침위중조표체고우전침신유조(P<0．05),전침신유조표체고우모형조(P<0．05),이재제14천,전침신유조표체현저고우모형조여전침위중조(P<0．01)。결론전침위중혈화전침신유혈균능구촉진대서요다렬기손상후적재생,전침위중혈재기육손상적조기효과현저。
Objective To observe the effect of electroacupuncture on multifidus musle injury by morphological observation and insulin like growth factor 1 ( IGF-1 ) expression of rats with bupivacaine in-duced multifidus muscle toxicity, and discuss the possible mechanism of muscle tissue regeneration. Meth-ods 120 male Sprague Dawley rats were randomly divided into blank group (BG), model control group (CG), model group (MG), Weizhong (BL40) electroacupuncture group (WG) and Shenshu(BL23) e-lectro-acupuncture group (SG), each group then was divided evenly into three time points (day4, day7 and day14) and each time point has 8 samples. Multifidus muscle (L4 and L5 sections) toxicity was estab-lished by injecting 0. 5% bupivacaine hydrochloride, and normal saline injection as control (CG). The WG and LG accepted electro-acupuncture for 4d, 7d or 14d after modeling. The multifidus muscle of each rodent was stained with HE to observe pathological changes, and immune-histochemical staining was used to observe IGF-1 expression. Results Bupivacaine caused a process of degeneration-regeneration of the muscle fibers and did not normalize at day 14. WG and SG were recovered faster than MG. The expression of IGF-1 at day 4 ranked as MG higher than BG (P<0. 01), WG higher than MG and SG (P<0. 01, P<0. 05), and SG higher than MG (P<0. 05), while ranked as SG higher than MG and WG (P<0. 01) at day14. Conclusion Electro-acupuncture at both BL40 and BL23 could promote the regeneration of tissue af-ter multifidus muscle injury, and it seems better therapy effect when electro-acupuncture at BL40.