CAJ | 학술논문

为探讨烟叶中叶黄素生物降解的方法，利用单因素试验和响应面分析法对叶黄素降解酶高产菌株Ewingella americana(Y32)的液体发酵工艺条件进行优化。结果表明，最适的碳源、氮源和无机盐分别为葡萄糖、酵母膏和KH2 PO4。在单因素试验的基础上，采用Box-Behnken试验设计进行回归分析得到最优培养基组成为：葡萄糖1.38%、酵母膏0.48%、KH2 PO40.19%，在该条件下叶黄素降解率为90.02%，与理论预测值(89.55%)基本吻合，比优化前的基础发酵培养基提高45.07%。
위탐토연협중협황소생물강해적방법，이용단인소시험화향응면분석법대협황소강해매고산균주Ewingella americana(Y32)적액체발효공예조건진행우화。결과표명，최괄적탄원、담원화무궤염분별위포도당、효모고화KH2 PO4。재단인소시험적기출상，채용Box-Behnken시험설계진행회귀분석득도최우배양기조성위：포도당1.38%、효모고0.48%、KH2 PO40.19%，재해조건하협황소강해솔위90.02%，여이론예측치(89.55%)기본문합，비우화전적기출발효배양기제고45.07%。
Optimization of lutein degradation enzyme production by Ewingella americana ( Y32 ) was investigated by response surface analysis methodology. First,the one-factor-at-a-time method was adopted to investigate the effects of variable of medium components( i. e. ,carbon, nitrogen,and mineral sources) on the enzyme production. Among these variables, glucose,yeast extract and KH2 PO4 were identified to be the most suitable carbon,nitrogen and inorganic salt,respectively. Subsequently,the concentration of glucose,yeast extract and KH2 PO4 were optimized using Box-Behnken regression analysis method. The optimal composition was found to be glucose 1. 38%, yeast extract 0. 48%, KH2 PO4 0. 19%. Under optimal culture conditions,the maximum degradation rate of lutein was 90. 02%,which was increased by 45. 07% compared with that before optimization. The experimental value was in according with that of predicting.