CAJ | 학술논문

根据已报道的小鼠核糖体蛋白L9基因( RPL9)序列设计PCR引物，克隆RPL9基因，并以β-actin为内参基因，采用相对荧光定量RT-PCR方法，检测、分析RPL9基因mRNA在小鼠心、肝、脾、肺、肾、脑及脂肪7个组织中表达量的差异。结果表明，成功克隆出RPL9基因序列全长，该基因在小鼠的7个组织中均有不同程度的转录表达，其中，在脂肪中的表达量最高，在肾、脾和脑中的表达量较高，在心、肝中的表达量较低，在肺中表达量最低。
근거이보도적소서핵당체단백L9기인( RPL9)서렬설계PCR인물，극륭RPL9기인，병이β-actin위내삼기인，채용상대형광정량RT-PCR방법，검측、분석RPL9기인mRNA재소서심、간、비、폐、신、뇌급지방7개조직중표체량적차이。결과표명，성공극륭출RPL9기인서렬전장，해기인재소서적7개조직중균유불동정도적전록표체，기중，재지방중적표체량최고，재신、비화뇌중적표체량교고，재심、간중적표체량교저，재폐중표체량최저。
We designed PCR primers which were based on ribosomal protein L9 gene ( RPL9 ) sequence in mouse reported,and then cloned RPL9 gene. Using β-actin as internal gene,the method of the relative fluorescence quantitative RT-PCR was used to detect and analyze the expression differences of RPL9 gene mRNA in seven kinds of tissues in mouse,such as heart,liver,spleen,lung,kidney,brain and fat. The re-sults showed that we cloned RPL9 gene successfully,the expression of RPL9 gene had different levels of transcription in different tissues,the highest amount of expression in the fat,higher expression in kidney, spleen and brain,lower amount of expression in the heart and liver,the lowest amount of expression in the lung.