CAJ | 학술논문

为了明确东北、华北地区致病疫霉群体的线粒体单倍型组成与多样性,采用PCR的方法对2013年采自两地区5省(区)的163株致病疫霉菌株的线粒体单倍型进行了测定和分析。由高变区HVRi所区分的Ⅰ型菌株所占比例为38.65%,Ⅱ型菌株占61.35%；高变区HVRii所区分的R1型、R2型和R3型菌株所占比例分别为1.85%、4.90%和93.25%。东北和华北两地区的优势线粒体单倍型均为ⅠR3型与ⅡR3型,其在群体中的比例分别为36.81%和56.44%。不同省(区)间致病疫霉线粒体单倍型组成存在一定差异,ⅡR2型菌株仅在河北和辽宁省有少量分布,所占比例仅为4.91%；ⅠR1型菌株仅存在于黑龙江,所占比例仅为1.84%。东北和华北地区致病疫霉线粒体单倍型整体表现一致,不同省(区)之间多态性不同,说明致病疫霉的线粒体单倍型与地理来源之间有很大关系。
위료명학동북、화북지구치병역매군체적선립체단배형조성여다양성,채용PCR적방법대2013년채자량지구5성(구)적163주치병역매균주적선립체단배형진행료측정화분석。유고변구HVRi소구분적Ⅰ형균주소점비례위38.65%,Ⅱ형균주점61.35%；고변구HVRii소구분적R1형、R2형화R3형균주소점비례분별위1.85%、4.90%화93.25%。동북화화북량지구적우세선립체단배형균위ⅠR3형여ⅡR3형,기재군체중적비례분별위36.81%화56.44%。불동성(구)간치병역매선립체단배형조성존재일정차이,ⅡR2형균주부재하북화요녕성유소량분포,소점비례부위4.91%；ⅠR1형균주부존재우흑룡강,소점비례부위1.84%。동북화화북지구치병역매선립체단배형정체표현일치,불동성(구)지간다태성불동,설명치병역매적선립체단배형여지리래원지간유흔대관계。
To understand the composition and polymorphism of mitochondrial haplotypes of Phytophthora infestans in Northeast and North China,one hundred and sixty three P. infestans isolates were collected from five provinces ( region) of the two areas in 2013 and their mitochondrial haplotypes were identified and analyzed by PCR. The results showed that the percentage of type Ⅰ was 38. 65% and the rest 61. 35% belonged to typeⅡ,which were distinguished by HVRi. The percentages of type R1 ,R2 and R3 , which were distinguished by HVRii,were 1. 85%,4. 90% and 93. 25%,respectively. Haplotypes ⅠR3 and ⅡR3 were dominant and widely distributed in the Northeast and the North China. The percentages of haplotypes ⅠR3 and ⅡR3 were 36. 81% and 56. 44%, respectively. The composition of mitochondrial haplotypes of P. infestans was weakly different in the tested five provinces (region). Only a few strains with ⅡR2 occurred in Hebei and Liaoning provinces,and its percentage was 4. 91%. HaplotypeⅠR1 only distributed in Heilongjiang province,and its percentage was 1. 84%. Mitochondrial haplotypes of P. infes-tans were similar in the Northeast and the North China, however their polymorphism was different in the five tested provinces(region). Therefore,the mitochondrial haplotypes of P. infestans had a certain rela-tionship to their geographical origin.