CAJ | 학술논문

采用 ELISA 法检测70例标本(肝癌组39例、肝硬化组31例)血清中磷脂酰肌醇蛋白聚糖3(GPC3)和高尔基体蛋白73(GP73)的水平,qRT-PCR 法检测相同标本外周血单个核细胞(PBMC)中 GPC3 mRNA 和 GP73 mRNA 的相对表达量。结果显示肝癌组血清中 GPC3和 GP73表达量显著高于肝硬化组(P <0．0001)；两组间 PBMC 中 GPC3 mRNA 和GP73 mRNA 水平差异无统计学意义；相关性分析结果显示,肝硬化组和肝癌组血清中 GPC3、GP73含量均有正相关性(P <0．01)。血清中 GPC3和 GP73的水平均能预测原发性肝癌,且 GPC3和 GP73的水平具有一定的相关性；PBMC 中GPC3 mRNA 和 GP73 mRNA 的表达水平不能很好的区分肝癌组与肝硬化组,提示 ELISA 法检测血清中 GPC3和 GP73的表达水平是临床预测原发性肝癌的首选方法。
채용 ELISA 법검측70례표본(간암조39례、간경화조31례)혈청중린지선기순단백취당3(GPC3)화고이기체단백73(GP73)적수평,qRT-PCR 법검측상동표본외주혈단개핵세포(PBMC)중 GPC3 mRNA 화 GP73 mRNA 적상대표체량。결과현시간암조혈청중 GPC3화 GP73표체량현저고우간경화조(P <0．0001)；량조간 PBMC 중 GPC3 mRNA 화GP73 mRNA 수평차이무통계학의의；상관성분석결과현시,간경화조화간암조혈청중 GPC3、GP73함량균유정상관성(P <0．01)。혈청중 GPC3화 GP73적수평균능예측원발성간암,차 GPC3화 GP73적수평구유일정적상관성；PBMC 중GPC3 mRNA 화 GP73 mRNA 적표체수평불능흔호적구분간암조여간경화조,제시 ELISA 법검측혈청중 GPC3화 GP73적표체수평시림상예측원발성간암적수선방법。
ELISA and qRT-PCR were used to detect the level of glypican3 (GPC3) and Golgi protein 73 (GP73) of 70 patients [primary hepatocellular carcinoma(HCC) group 39 patients and cirrhosis group 31 patients] in ser-um and peripheral blood mononuclear(PBMC) respectively. The results showed that the expression levels of GPC3 and GP73 in serum had significant difference between two groups(P < 0. 001), but the relative expression of GPC3 and GP73 mRNA in PBMC had no great difference. Correlation analysis showed that the expression levels of GPC3 and GP73 in serum were positively correlated (P < 0. 01). In conclusion, the expression levels of GPC3 and GP73 in serum can predict HCC, and the levels of GPC3 and GP73 have some relevance. GPC3 and GP73 mRNA levels in PBMC can′t distinguish between two groups, suggesting ELISA is the preferred method of clinical to detect the expression levels of GPC3 and GP3 in serum of HCC.