医学信息
醫學信息
의학신식
MEDICAL INFORMATION
2015年
18期
12-13
,共2页
朱小凤%莫似恩%梁志锋%唐%林耀旺%杨晓梅%刘林%陆璐%张璇%曾广柳%伍朝强%梁佩艳%唐悦%吴虹欢
硃小鳳%莫似恩%樑誌鋒%唐%林耀旺%楊曉梅%劉林%陸璐%張璇%曾廣柳%伍朝彊%樑珮豔%唐悅%吳虹歡
주소봉%막사은%량지봉%당%림요왕%양효매%류림%륙로%장선%증엄류%오조강%량패염%당열%오홍환
子宫内膜容受性%白血病抑制因子%体外受精-胚胎移植%不孕症%子宫内膜%子宫内膜息肉%苏木素-伊红染色%免疫组织化学染色
子宮內膜容受性%白血病抑製因子%體外受精-胚胎移植%不孕癥%子宮內膜%子宮內膜息肉%囌木素-伊紅染色%免疫組織化學染色
자궁내막용수성%백혈병억제인자%체외수정-배태이식%불잉증%자궁내막%자궁내막식육%소목소-이홍염색%면역조직화학염색
Receptivity of endometrium%Leukemia inhibitory factor(LIF)%Vitro fertilization-embryo transfer(IVF-ET)%Infertility%Endometrium%Endometrial Polyps(EP)%Hematoxylin-Eosin(HE) Staining%Immunohistochemical staining
目的通过收集在广西生殖健康中心行体外受精-胚胎移植(IVF-ET)不孕症妇女子宫内膜和息肉组织做病理切片苏木素-伊红(HE.)染色和白血病抑制因子(LIF)免疫组化染色，观察LIF表达分布情况，为研究提高子宫内膜息肉(EP)不孕症患者妊娠率提供参考依据。方法在广西生殖健康中心收集30例确诊为EP不孕症并行IVF-ET患者的子宫内膜和息肉作研究组，另取30例同期在本中心以丈夫不育为主因行IVF-ET的非EP患者(接近正常)子宫内膜组织作正常对照组，同期再在广西医科大学一附院门诊收集30例非息肉、非不孕症的放取环诊刮患者子宫内膜组织作非IVF-ET对照组，分别进行病理切片H.E染色及LIF免疫组织化学染色。结果与对照组比较，研究组内膜主要为腺泡细胞胞浆强阳性表达而胞核不表达；腺腔分泌物及间质细胞胞浆也有一定阳性表达；研究组息肉散在小片状间质细胞胞浆强阳性表达，腺细胞基本无阳性表达。结论广西IVE-ET的EP不孕患者子宫内膜LIF在腺上皮胞浆强阳性表达而胞核不表达，息肉组织则间质部分表达而腺细胞不表达。
目的通過收集在廣西生殖健康中心行體外受精-胚胎移植(IVF-ET)不孕癥婦女子宮內膜和息肉組織做病理切片囌木素-伊紅(HE.)染色和白血病抑製因子(LIF)免疫組化染色，觀察LIF錶達分佈情況，為研究提高子宮內膜息肉(EP)不孕癥患者妊娠率提供參攷依據。方法在廣西生殖健康中心收集30例確診為EP不孕癥併行IVF-ET患者的子宮內膜和息肉作研究組，另取30例同期在本中心以丈伕不育為主因行IVF-ET的非EP患者(接近正常)子宮內膜組織作正常對照組，同期再在廣西醫科大學一附院門診收集30例非息肉、非不孕癥的放取環診颳患者子宮內膜組織作非IVF-ET對照組，分彆進行病理切片H.E染色及LIF免疫組織化學染色。結果與對照組比較，研究組內膜主要為腺泡細胞胞漿彊暘性錶達而胞覈不錶達；腺腔分泌物及間質細胞胞漿也有一定暘性錶達；研究組息肉散在小片狀間質細胞胞漿彊暘性錶達，腺細胞基本無暘性錶達。結論廣西IVE-ET的EP不孕患者子宮內膜LIF在腺上皮胞漿彊暘性錶達而胞覈不錶達，息肉組織則間質部分錶達而腺細胞不錶達。
목적통과수집재엄서생식건강중심행체외수정-배태이식(IVF-ET)불잉증부녀자궁내막화식육조직주병리절편소목소-이홍(HE.)염색화백혈병억제인자(LIF)면역조화염색，관찰LIF표체분포정황，위연구제고자궁내막식육(EP)불잉증환자임신솔제공삼고의거。방법재엄서생식건강중심수집30례학진위EP불잉증병행IVF-ET환자적자궁내막화식육작연구조，령취30례동기재본중심이장부불육위주인행IVF-ET적비EP환자(접근정상)자궁내막조직작정상대조조，동기재재엄서의과대학일부원문진수집30례비식육、비불잉증적방취배진괄환자자궁내막조직작비IVF-ET대조조，분별진행병리절편H.E염색급LIF면역조직화학염색。결과여대조조비교，연구조내막주요위선포세포포장강양성표체이포핵불표체；선강분비물급간질세포포장야유일정양성표체；연구조식육산재소편상간질세포포장강양성표체，선세포기본무양성표체。결론엄서IVE-ET적EP불잉환자자궁내막LIF재선상피포장강양성표체이포핵불표체，식육조직칙간질부분표체이선세포불표체。
Objective To observe the expression and distribution of Leukemia Inhibitory Factor(LIF) Immunohistochemical staining in endometrium and Endometrial Polyp(EP) tissues who performed In Vitro Fertilization-Embryo Transfer (IVF-ET) in Guangxi Reproductive Health Center (GXRHC), so that to provide the reference for researching how to improve pregnancy rate in EP patients with infertility. Methods Col ected 30 patients who was diagnosed with EP infertility and planed perform IVF-ET in GXRHC as a research group, meanwhile, the other similar 30 cases were male factor (husband infertility) but no EP and close to the normal as a normal control group, 30 cases were col ected in the family planning outpatients of First Af iliated Hospital of Guangxi Medical University who put in or take out an intrauterine device (IUD) as a Nor IVF-ET control group. Al the endometrium or endometrial polyp tissues and specimens were obtained by hysteroscopic polypectomy or curet age surgery, and were done HE staining and LIF immunohistochemical staining, and then observed the expression of LIF. Results Compared with the control group, glandular cel s cytoplasm strong positive expression and the nuclei did not express; Lumens secretions and interstitial cel cytoplasm also to have a certain positive expression in a research group; a few smal region interstitial cel cytoplasm strong positive expression, but glandular cel almost no positive expression. Conclusion Glandular cel s cytoplasm higher positive expressed but the nuclei did not express with LIF in endometrium, and interstitial cel s partly positive expressed but glandular cel s did not express in Endometrial Polyps tissues of EP infertility women who perform IVE-ET in Guangxi.
