CAJ | 학술논문

为了优化甘蓝型油菜小孢子培养技术,提高胚诱导率及成苗率,以油菜N14009为试验材料进行小孢子培养,研究变温预处理、活性炭浓度、培养基类型和琼脂浓度及秋水仙碱浓度对小孢子培养的影响。结果表明：4℃、32.5℃变温预处理各1 d的处理效果较好,胚诱导率为4.23胚/蕾；活性炭质量浓度为0.5 g/L时,胚诱导率达到4.23胚/蕾,效果最好； MS、B5培养基中琼脂质量浓度为12 g/L时,成苗率均最高,分别达51.16%、46.67%；把再生植株转到含有不同质量浓度秋水碱的MS培养基上培养,质量浓度为80 mg/L时,N14009的加倍率最高,为43.47%,并且成苗率达到76.67%。
위료우화감람형유채소포자배양기술,제고배유도솔급성묘솔,이유채N14009위시험재료진행소포자배양,연구변온예처리、활성탄농도、배양기류형화경지농도급추수선감농도대소포자배양적영향。결과표명：4℃、32.5℃변온예처리각1 d적처리효과교호,배유도솔위4.23배/뢰；활성탄질량농도위0.5 g/L시,배유도솔체도4.23배/뢰,효과최호； MS、B5배양기중경지질량농도위12 g/L시,성묘솔균최고,분별체51.16%、46.67%；파재생식주전도함유불동질량농도추수감적MS배양기상배양,질량농도위80 mg/L시,N14009적가배솔최고,위43.47%,병차성묘솔체도76.67%。
In order to optimize microspore culture techniques of Brassica napus L. and improve the embryo induction rate and seedling rate, the N14009 was used as experimental material to culture microspores. During the research,the effects on microspore culture were studied by different temperature pretreatment,concentration of activated carbon,medium type and agar concentration and concentration of colchicine . The results showed that:It got a good result by 4℃ and 32 . 5℃ temperature pretreatments for one day each,and the embryo induction rate was 4. 23 embryos/bud;When the concentration of activated carbon was 0. 5 g/L,the embryo induction rate reached the maximum,4. 23 embryos/bud;In addition,the seedling rate reached 51. 16% and 46. 67% in the MS and B5 media both with 12 g/L of agar, respectively. The regenerated plants were turned to MS that had different concentration of colchicine, the doubling efficiency of N14009 reached 43 . 47% and the seedling rate reached 76 . 67%, when the concentration of colchicine was 80 mg/L.