CAJ | 학술논문

目的：通过实时定量逆转录 PCR(RT-PCR)、肾脏免疫组化和 ELISA 3种方法研究儿童狼疮性肾炎(LN)中 EB病毒(EBV)潜伏膜蛋白1(LMP-1)的表达,试图探讨 LMP-1在儿童 LN 中的致病机制。方法41例 LN 患儿和12例健康对照组,分别用 RT-PCR 法、免疫组化法、ELISA 法检测两组外周血单核细胞(PBMCs)、肾脏组织及血清中 LMP-1的表达。结果① RT-PCR 法、免疫组化法、ELISA 法检测 LN患儿和健康对照者的阳性率分别为65．9% vs 8．3%,95．1%vs 16．7%,22．0% vs 8．3%,差异有统计学意义(P <0．05)；②3种方法检测 LMP-1的表达,免疫组化法检测阳性率高于其他两种方法,差异有统计学意义(P <0．05)。结论儿童 LN 中存在 EBV 潜伏期基因 LMP-1表达异常,其中肾脏组织中表达阳性率最高,提示 LMP-1可能参与了儿童 LN 的发病。
목적：통과실시정량역전록 PCR(RT-PCR)、신장면역조화화 ELISA 3충방법연구인동랑창성신염(LN)중 EB병독(EBV)잠복막단백1(LMP-1)적표체,시도탐토 LMP-1재인동 LN 중적치병궤제。방법41례 LN 환인화12례건강대조조,분별용 RT-PCR 법、면역조화법、ELISA 법검측량조외주혈단핵세포(PBMCs)、신장조직급혈청중 LMP-1적표체。결과① RT-PCR 법、면역조화법、ELISA 법검측 LN환인화건강대조자적양성솔분별위65．9% vs 8．3%,95．1%vs 16．7%,22．0% vs 8．3%,차이유통계학의의(P <0．05)；②3충방법검측 LMP-1적표체,면역조화법검측양성솔고우기타량충방법,차이유통계학의의(P <0．05)。결론인동 LN 중존재 EBV 잠복기기인 LMP-1표체이상,기중신장조직중표체양성솔최고,제시 LMP-1가능삼여료인동 LN 적발병。
Objective To discuss the role of latent membrane protein 1 (LMP-1)in the pathgenesis of juvenile lu-pus nephritis ( LN) through investigating expression of LMP-1 by RT-PCR, immunohistochemistry and ELISA. Methods RT-PCR,immunohistochemistry and ELISA were applied to detect expression of LMP-1 in PBMCs, kid-ney tissue and serum from 20 patients with LN and 12 healthy control subjects respectively. Results ① There was signifcant difference between juvenile LN patients and healthy controls by RT-PCR, immunohistochemistry and ELISA,Positive rate were 65. 9% vs 8. 3% ,95. 1% vs 16. 7% ,22. 0% vs 8. 3% , respectively(P < 0. 05). ② Pos-itive rates of LMP-1 in juvenile LN were significantly different among three methods and the positive rate detected by immunohistochemistry was higher than the other two methods (P < 0. 05). Conclusion The results demonstrate that there are aberrant expression of LMP-1 gene in juvenile LN, thepositive rate of LMP-1 in kidney tissue is high-est, suggesting that LMP-1 gene may contribute to the pathgenesis of juvenile LN.