CAJ | 학술논문

目的：观察塞来昔布对人成骨细胞增殖的影响及其作用机制。方法将人成骨细胞分为3个组：空白对照组、IL-1刺激组(阴性对照组)、塞来昔布+ IL-1刺激组(药物干预组)，用 RT-PCR 法和 Western blot 法分别检测三组成骨细胞环氧化酶-2( COX-2)、膜相关前列腺素 E2合成酶1(mPGES-1) mRNA 和蛋白的表达。 MTT 法检测塞来昔布对阴性对照组人成骨细胞增殖的影响。结果 RT-PCR 法和Western blot 法实验结果显示，同空白对照组相比，阴性对照组的人成骨细胞中 COX-2、mPGES-1 mRNA 和蛋白的表达均增加；同阴性对照组相比，药物干预组的 COX-2、mPGES-1 mRNA 和蛋白的表达均降低。塞来昔布对 IL-1刺激的人成骨细胞的生长具有抑制作用，呈现剂量依赖性，浓度越大，抑制作用越大；并且随着作用时间的延长，细胞的抑制作用增大。结论塞来昔布可以抑制炎性状态下人成骨细胞的生长，可能是通过调控 COX-2、mPGES-1 mRNA 和蛋白的表达，使其表达下调而发挥作用。
목적：관찰새래석포대인성골세포증식적영향급기작용궤제。방법장인성골세포분위3개조：공백대조조、IL-1자격조(음성대조조)、새래석포+ IL-1자격조(약물간예조)，용 RT-PCR 법화 Western blot 법분별검측삼조성골세포배양화매-2( COX-2)、막상관전렬선소 E2합성매1(mPGES-1) mRNA 화단백적표체。 MTT 법검측새래석포대음성대조조인성골세포증식적영향。결과 RT-PCR 법화Western blot 법실험결과현시，동공백대조조상비，음성대조조적인성골세포중 COX-2、mPGES-1 mRNA 화단백적표체균증가；동음성대조조상비，약물간예조적 COX-2、mPGES-1 mRNA 화단백적표체균강저。새래석포대 IL-1자격적인성골세포적생장구유억제작용，정현제량의뢰성，농도월대，억제작용월대；병차수착작용시간적연장，세포적억제작용증대。결론새래석포가이억제염성상태하인성골세포적생장，가능시통과조공 COX-2、mPGES-1 mRNA 화단백적표체，사기표체하조이발휘작용。
Objective To study the impact of celecoxib on proliferation of human osteoblasts. Methods The hu-man osteoblasts were cultured and divided into three groups,including the negative control, positive control (stimu-lated by IL-1) and intervention group (celecoxib + IL-1). The detection of mRNA and protein level of COX-2 and mPGES-1 were measured by real tmie PCR (RT-PCR) and Western blot. MTT assay was performed to determine the impact of celecoxib on proliferation of human osteoblasts which were treated with IL-1. Results RT-PCR and Western blot showed that the detection of mRNA and protein level of COX-2 and mPGES-1 of the positive control (stimulated by IL-1) were significantly higher than that of the negative control and intervention group (celecoxib +IL-1). Celecoxib had cytotoxic effect on the growth of osteoblasts, manifests dose dependence as well as increasing cell inhibition over time. Conclusion Celecoxib could inhibit proliferation of osteoblasts, and could inhibit the oc-currence of heterotopic ossification by regulating the level of mPGES-1 and COX-2 mRNA and protein.