安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2015年
5期
581-584
,共4页
文剑明%王锐英%高燕%胡译文%陶波%周文静
文劍明%王銳英%高燕%鬍譯文%陶波%週文靜
문검명%왕예영%고연%호역문%도파%주문정
TGF-β1%骨髓间充质干细胞%电转染%Ⅱ型胶原
TGF-β1%骨髓間充質榦細胞%電轉染%Ⅱ型膠原
TGF-β1%골수간충질간세포%전전염%Ⅱ형효원
transforming growth factor beta 1%mesenchymal stem cells%electropration%collagen Ⅱ
目的:通过电转染介导转化生长因子β1(TGF-β1)转染兔骨髓间充质干细胞(BMSCs),观察Ⅱ型胶原表达的情况。方法用全骨髓细胞贴壁培养法分离、培养兔 BMSCs;诱导14 d 后,免疫组化和 Western blot 法检测Ⅱ型胶原表达。结果 BMSCs CD90表达阳性,CD31表达阴性;成功转染 TGF-β1至 BMSCs;通过免疫组化及 Western blot 法检测TGF-β1组细胞内Ⅱ型胶原有较强的表达,与增强型绿色荧光蛋白(EGFP)组和空白对照组相比差异有统计学意义(P<0.05)。结论电转 TGF-β1质粒至兔 BMSCs,可以促进Ⅱ型胶原表达。
目的:通過電轉染介導轉化生長因子β1(TGF-β1)轉染兔骨髓間充質榦細胞(BMSCs),觀察Ⅱ型膠原錶達的情況。方法用全骨髓細胞貼壁培養法分離、培養兔 BMSCs;誘導14 d 後,免疫組化和 Western blot 法檢測Ⅱ型膠原錶達。結果 BMSCs CD90錶達暘性,CD31錶達陰性;成功轉染 TGF-β1至 BMSCs;通過免疫組化及 Western blot 法檢測TGF-β1組細胞內Ⅱ型膠原有較彊的錶達,與增彊型綠色熒光蛋白(EGFP)組和空白對照組相比差異有統計學意義(P<0.05)。結論電轉 TGF-β1質粒至兔 BMSCs,可以促進Ⅱ型膠原錶達。
목적:통과전전염개도전화생장인자β1(TGF-β1)전염토골수간충질간세포(BMSCs),관찰Ⅱ형효원표체적정황。방법용전골수세포첩벽배양법분리、배양토 BMSCs;유도14 d 후,면역조화화 Western blot 법검측Ⅱ형효원표체。결과 BMSCs CD90표체양성,CD31표체음성;성공전염 TGF-β1지 BMSCs;통과면역조화급 Western blot 법검측TGF-β1조세포내Ⅱ형효원유교강적표체,여증강형록색형광단백(EGFP)조화공백대조조상비차이유통계학의의(P<0.05)。결론전전 TGF-β1질립지토 BMSCs,가이촉진Ⅱ형효원표체。
Objective To observe cases of type Ⅱ collagen expression after transforming growth factor-β1 (TGF-β1) infected in rabbit bone mesenchymal stem cells( BMSCs) by electropration. Methods BMSCs from rabbit were isolated and cultured. 14 days after induction, immunohistochemistry and Western blot methods type Ⅱ colla-gen were used to detect the expression of type Ⅱ collagen. Results CD90 was positive and CD31 was negative by flow cytometry;successfully transfected with TGF-β1 to the BMSCs,the strong expression of type Ⅱ collagen in TGF-β1 group cells was shown by Western blot and immunocy-tochemical stain, which was compared with empty plasmid group and the control group,and the difference was statistically significant(P < 0. 05). Conclusion TGF-β1 plasmid by electroporation to rabbit BMSCs can facilitate expression of collagen Ⅱ.