安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2015年
5期
569-572
,共4页
李娟%曹云霞%刘婷婷%许孝凤
李娟%曹雲霞%劉婷婷%許孝鳳
리연%조운하%류정정%허효봉
卵丘细胞%长链非编码 RNA%ENST00000502521%胚胎发育
卵丘細胞%長鏈非編碼 RNA%ENST00000502521%胚胎髮育
란구세포%장련비편마 RNA%ENST00000502521%배태발육
cumulus cells%long noncoding RNAs%ENST00000502521%embryo developmental competence
目的:探讨体外受精(IVF)周期人卵丘细胞(CCs)中lncRNA ENST00000502521的表达与卵母细胞成熟及胚胎发育的关系,判断其对体外受精-胚胎移植(IVF-ET)结局的预测价值。方法收集40例首次接受长方案超促排卵患者的卵丘细胞,根据卵丘细胞围绕的卵母细胞受精后胚胎发育情况将卵丘细胞分成两组,优质胚胎组和低质胚胎组,采用实时荧光定量逆转录-聚合酶链反应(qRT-PCR)技术检测两组卵丘细胞中 lncRNA ENST00000502521的相对表达量,分析其与胚胎发育及妊娠结局的关系;采用受试者工作特征曲线(ROC)评价 lncRNA ENST00000502521相对表达量对临床妊娠结局的预测价值。结果 IVF 患者低质胚胎组中 ln-cRNA ENST0000050252表达较优质胚胎组明显增高( P <0.05),且未妊娠组 lncRNA ENST00000502521相对表达量较妊娠组增高(P <0.05);lncRNA ENST00000502521预测妊娠结局的 ROC 曲线下面积(AUC)为0.71(P <0.05,95% CI:0.55~0.87),灵敏度为84.2%,特异性为61.9%。结论lncRNA ENST00000502521可能参与影响卵母细胞成熟及胚胎发育,可作为一个潜在的分子标志物预测胚胎质量和妊娠结局。
目的:探討體外受精(IVF)週期人卵丘細胞(CCs)中lncRNA ENST00000502521的錶達與卵母細胞成熟及胚胎髮育的關繫,判斷其對體外受精-胚胎移植(IVF-ET)結跼的預測價值。方法收集40例首次接受長方案超促排卵患者的卵丘細胞,根據卵丘細胞圍繞的卵母細胞受精後胚胎髮育情況將卵丘細胞分成兩組,優質胚胎組和低質胚胎組,採用實時熒光定量逆轉錄-聚閤酶鏈反應(qRT-PCR)技術檢測兩組卵丘細胞中 lncRNA ENST00000502521的相對錶達量,分析其與胚胎髮育及妊娠結跼的關繫;採用受試者工作特徵麯線(ROC)評價 lncRNA ENST00000502521相對錶達量對臨床妊娠結跼的預測價值。結果 IVF 患者低質胚胎組中 ln-cRNA ENST0000050252錶達較優質胚胎組明顯增高( P <0.05),且未妊娠組 lncRNA ENST00000502521相對錶達量較妊娠組增高(P <0.05);lncRNA ENST00000502521預測妊娠結跼的 ROC 麯線下麵積(AUC)為0.71(P <0.05,95% CI:0.55~0.87),靈敏度為84.2%,特異性為61.9%。結論lncRNA ENST00000502521可能參與影響卵母細胞成熟及胚胎髮育,可作為一箇潛在的分子標誌物預測胚胎質量和妊娠結跼。
목적:탐토체외수정(IVF)주기인란구세포(CCs)중lncRNA ENST00000502521적표체여란모세포성숙급배태발육적관계,판단기대체외수정-배태이식(IVF-ET)결국적예측개치。방법수집40례수차접수장방안초촉배란환자적란구세포,근거란구세포위요적란모세포수정후배태발육정황장란구세포분성량조,우질배태조화저질배태조,채용실시형광정량역전록-취합매련반응(qRT-PCR)기술검측량조란구세포중 lncRNA ENST00000502521적상대표체량,분석기여배태발육급임신결국적관계;채용수시자공작특정곡선(ROC)평개 lncRNA ENST00000502521상대표체량대림상임신결국적예측개치。결과 IVF 환자저질배태조중 ln-cRNA ENST0000050252표체교우질배태조명현증고( P <0.05),차미임신조 lncRNA ENST00000502521상대표체량교임신조증고(P <0.05);lncRNA ENST00000502521예측임신결국적 ROC 곡선하면적(AUC)위0.71(P <0.05,95% CI:0.55~0.87),령민도위84.2%,특이성위61.9%。결론lncRNA ENST00000502521가능삼여영향란모세포성숙급배태발육,가작위일개잠재적분자표지물예측배태질량화임신결국。
Objective To explore the expression of lncRNA ENST00000502521 in human cumulus cells of women undergoing in vitro fertilization (IVF) and the relationship between lncRNA ENST00000502521 and oocyte matura-tion and embryo development. Moreover, its predictive value of IVF outcome was also discussed. Methods The expression of lncRNA ENST00000502521 was measured in 40 pairs of cumulus cells from oocytes resulting in high-quality embryo and from oocytes resulting in poor-quality embryo by real-time quantitative reverse transcriptase-pol-ymerase chain reaction (qRT-PCR), and further associated these data with embryo developmental potential and competence to establish a pregnancy. The predictive value of the lncRNA ENST00000502521 was evaluated using a receiver operating characteristic (ROC) curve. Results Notably elevated lncRNA ENST00000502521 expression levels were observed in the group poor-quality embryo compared to the group high-quality embryo (P < 0. 05). The relative expression levels of lncRNA ENST00000502521 in the non-pregnancy group were significantly higher than those in the pregnancy group (P < 0. 05). The area under the ROC curve(AUC) of lncRNA ENST00000502521 was 0. 71 (P < 0. 05,95% CI: 0. 55 ~ 0. 87) and had a sensitivity of 84. 2% and a specificity of 61. 9% to predict embryo developmental potential. Conclusion The relative expression levels of lncRNA ENST00000502521 may be considered as potential biomarkers to predict embryo developmental competence and IVF outcome.