CAJ | 학술논문

目的：观察大鼠帕金森病（Parkinson’disease ，PD）水通道蛋白9（aquaporin 9，AQP9）在脑组织中的表达变化，研究AQP9在帕金森病脑组织发病机制中的作用。方法制造帕金森病动物模型，应用HE染色、免疫组织化学、RT‐PCR等方法观察大鼠脑组织AQP9表达变化。结果免疫组织化学和RT‐PCR检测结果显示，帕金森病组脑组织 AQP9和AQP9 mRNA 的表达呈动态变化过程，帕金森病组大鼠术后各时间点（除外术后2周时间点）在鼠左侧黑质致密部（SNC ）及中脑腹侧被盖区（VTA）区AQP9和AQP9 mRNA表达均比假手术组高。结论高表达的AQP9，可能通过调节水电解质与能量代谢，从而在帕金森病发病机制中起重要作用。
목적：관찰대서파금삼병（Parkinson’disease ，PD）수통도단백9（aquaporin 9，AQP9）재뇌조직중적표체변화，연구AQP9재파금삼병뇌조직발병궤제중적작용。방법제조파금삼병동물모형，응용HE염색、면역조직화학、RT‐PCR등방법관찰대서뇌조직AQP9표체변화。결과면역조직화학화RT‐PCR검측결과현시，파금삼병조뇌조직 AQP9화AQP9 mRNA 적표체정동태변화과정，파금삼병조대서술후각시간점（제외술후2주시간점）재서좌측흑질치밀부（SNC ）급중뇌복측피개구（VTA）구AQP9화AQP9 mRNA표체균비가수술조고。결론고표체적AQP9，가능통과조절수전해질여능량대사，종이재파금삼병발병궤제중기중요작용。
Objective To observe the expression changes of aquapor‐9 ( AQP9) and its role in rat brain tissues of Parkin‐son’s disease (PD).Methods Parkinson's disease model in rats was made. The expression changes of AQP9 was detected by HE stain ,immunohistochemistry method and RT‐PCR.Results The detection of immunohistochemistry and RT‐PCR showed that the expressions of AQP9 and AQP9 mRNA presented dynamic changing process in Parkinson's disease group. The expres‐sions of AQP9 and AQP9 mRNA in the positions of left substantia nigra pars compacta (SNC) and the ventral tegmental area (VTA) in Parkinson's disease group were higher than that in the sham operation group at each time point after operation (ex‐cept for at 2‐week time point). Conclusion The high expression of AQP9 possibly plays an important role in the pathogenesis of Parkinson's disease by regulation of water electrolyte and energy metabolism.