中外医疗
中外醫療
중외의료
CHINA FOREIGN MEDICAL TREATMENT
2015年
10期
10-11
,共2页
结核病%结核分歧杆菌%血清学%HSP65
結覈病%結覈分歧桿菌%血清學%HSP65
결핵병%결핵분기간균%혈청학%HSP65
Tuberculosis%Mycobacterium tuberculosis%Serology%HSP65
目的:通过评价HSP65抗原表达在结核病血清诊断中的效果,分析其应用前景。方法取活动期结核病患者63例纳入观察组,选取体检健康志愿者60例纳入对照组,就HSP65分别与38 kDa、16 kDa、LAM联合检测诊断对照组与观察组结核病。结果Western-blot结果显示,表达产物在相对分子量65 kμ处有一条表达带,在健康人血清阴性中则无表达带;特异度、敏感度与38 kPa基本一致,差异无统计学意义(P>0.05)。结论 HSP65在结核病血清诊断中可作为备选标志物,反应强度与38kDa基本相当,联合诊断有助于提高诊断符合率;HSP65对结核病X线空洞影敏感度较高。
目的:通過評價HSP65抗原錶達在結覈病血清診斷中的效果,分析其應用前景。方法取活動期結覈病患者63例納入觀察組,選取體檢健康誌願者60例納入對照組,就HSP65分彆與38 kDa、16 kDa、LAM聯閤檢測診斷對照組與觀察組結覈病。結果Western-blot結果顯示,錶達產物在相對分子量65 kμ處有一條錶達帶,在健康人血清陰性中則無錶達帶;特異度、敏感度與38 kPa基本一緻,差異無統計學意義(P>0.05)。結論 HSP65在結覈病血清診斷中可作為備選標誌物,反應彊度與38kDa基本相噹,聯閤診斷有助于提高診斷符閤率;HSP65對結覈病X線空洞影敏感度較高。
목적:통과평개HSP65항원표체재결핵병혈청진단중적효과,분석기응용전경。방법취활동기결핵병환자63례납입관찰조,선취체검건강지원자60례납입대조조,취HSP65분별여38 kDa、16 kDa、LAM연합검측진단대조조여관찰조결핵병。결과Western-blot결과현시,표체산물재상대분자량65 kμ처유일조표체대,재건강인혈청음성중칙무표체대;특이도、민감도여38 kPa기본일치,차이무통계학의의(P>0.05)。결론 HSP65재결핵병혈청진단중가작위비선표지물,반응강도여38kDa기본상당,연합진단유조우제고진단부합솔;HSP65대결핵병X선공동영민감도교고。
Objective To analyze the application prospect of HSP65 antigen expression in serum by evaluating its value in the diagnosis of tuberculosis. Methods 63 cases with tuberculosis in active stage were included in the observation group, and 60 healthy volunteers underwent physical examination were enrolled in the control group. The combined detection of HSP65 with 38 kDa, 16 kDa, LAM was performed in both groups respectively for the diagnosis of tuberculosis. Results The result of Western-blot showed that the expressed product had an expression belt at the relative molecular weight of 65 kμ, while that had no expression belt in the negative serum of healthy people. The specificity and sensitivity were basically in line with 38kPa, the difference was not statistically significant(P>0.05). Conclusion HSP65 can be used as a candidate marker in serum for the diagnosis of tuberculosis, its intensity of reaction is roughly similar to 38kDa, the combined diagnosis is helpful to improve the diagnosis rate. HSP65 is hypersensitive to tuberculosis X-ray empty shadow.