中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2015年
5期
491-496
,共6页
马春星%唐铁轮%陈启仪%倪玲%姜军%李宁
馬春星%唐鐵輪%陳啟儀%倪玲%薑軍%李寧
마춘성%당철륜%진계의%예령%강군%리저
慢传输型便秘%动物模型,大鼠%苯扎氯铵%5-羟色胺%Cajal间质细胞
慢傳輸型便祕%動物模型,大鼠%苯扎氯銨%5-羥色胺%Cajal間質細胞
만전수형편비%동물모형,대서%분찰록안%5-간색알%Cajal간질세포
Slow transit constipation%Animal model,rats%Benzalkonium chloride%5-hydroxytryptamine%Interstitial cells of Cajal
目的:通过结肠神经节消融术建立一种新型大鼠慢传输型便秘模型。方法选用5~6周龄雌性大鼠,分为正常对照组、假手术组、实验Ⅰ、Ⅱ、Ⅲ和Ⅳ组,每组8只,正常对照组不做处理,假手术组及实验组大鼠在麻醉下,通过腹腔手术分别给予生理盐水及0.05%、0.1%、0.25%、0.5%苯扎氯铵溶液处理全结肠30 min。术后2周开始记录各组大鼠24 h排粪粒数、粪便干重和胃肠传输时间,并通过苏木精-伊红染色、免疫组织化学染色和酶联免疫吸附等方法对结肠病理改变、肠道神经细胞、Cajal间质细胞、神经递质5-羟色胺(5-HT)浓度进行评价。结果实验Ⅱ组和实验Ⅲ组与假手术组相比,粪便重量明显减少,胃肠运输时间明显延长(P<0.05)。苏木精-伊红染色及免疫组织化学染色结果显示,实验组大鼠肠肌层神经细胞减少,黏膜层出现不同程度炎性细胞浸润,环形肌变薄,黏膜下层Cajal间质细胞减少,符合慢传输型便秘病理改变。实验Ⅲ组5-HT浓度较其他组明显降低(P<0.01)。结论采用0.25%苯扎氯铵消融结肠神经节可成功构建结肠慢传输型便秘动物模型,该模型制作简单、结果稳定且较为符合慢传输型便秘病理改变。
目的:通過結腸神經節消融術建立一種新型大鼠慢傳輸型便祕模型。方法選用5~6週齡雌性大鼠,分為正常對照組、假手術組、實驗Ⅰ、Ⅱ、Ⅲ和Ⅳ組,每組8隻,正常對照組不做處理,假手術組及實驗組大鼠在痳醉下,通過腹腔手術分彆給予生理鹽水及0.05%、0.1%、0.25%、0.5%苯扎氯銨溶液處理全結腸30 min。術後2週開始記錄各組大鼠24 h排糞粒數、糞便榦重和胃腸傳輸時間,併通過囌木精-伊紅染色、免疫組織化學染色和酶聯免疫吸附等方法對結腸病理改變、腸道神經細胞、Cajal間質細胞、神經遞質5-羥色胺(5-HT)濃度進行評價。結果實驗Ⅱ組和實驗Ⅲ組與假手術組相比,糞便重量明顯減少,胃腸運輸時間明顯延長(P<0.05)。囌木精-伊紅染色及免疫組織化學染色結果顯示,實驗組大鼠腸肌層神經細胞減少,黏膜層齣現不同程度炎性細胞浸潤,環形肌變薄,黏膜下層Cajal間質細胞減少,符閤慢傳輸型便祕病理改變。實驗Ⅲ組5-HT濃度較其他組明顯降低(P<0.01)。結論採用0.25%苯扎氯銨消融結腸神經節可成功構建結腸慢傳輸型便祕動物模型,該模型製作簡單、結果穩定且較為符閤慢傳輸型便祕病理改變。
목적:통과결장신경절소융술건립일충신형대서만전수형편비모형。방법선용5~6주령자성대서,분위정상대조조、가수술조、실험Ⅰ、Ⅱ、Ⅲ화Ⅳ조,매조8지,정상대조조불주처리,가수술조급실험조대서재마취하,통과복강수술분별급여생리염수급0.05%、0.1%、0.25%、0.5%분찰록안용액처리전결장30 min。술후2주개시기록각조대서24 h배분립수、분편간중화위장전수시간,병통과소목정-이홍염색、면역조직화학염색화매련면역흡부등방법대결장병리개변、장도신경세포、Cajal간질세포、신경체질5-간색알(5-HT)농도진행평개。결과실험Ⅱ조화실험Ⅲ조여가수술조상비,분편중량명현감소,위장운수시간명현연장(P<0.05)。소목정-이홍염색급면역조직화학염색결과현시,실험조대서장기층신경세포감소,점막층출현불동정도염성세포침윤,배형기변박,점막하층Cajal간질세포감소,부합만전수형편비병리개변。실험Ⅲ조5-HT농도교기타조명현강저(P<0.01)。결론채용0.25%분찰록안소융결장신경절가성공구건결장만전수형편비동물모형,해모형제작간단、결과은정차교위부합만전수형편비병리개변。
Objective To establish an innovative rat model of slow transit constipation by selective chemical ablation of the colon enteric plexus. Methods Sprague Dawley rats, 5-6 weeks old, were randomly divided into normal control group, sham operation group, treatment groupⅠ, Ⅱ, Ⅲ,Ⅳ. The normal control group did not receive treatment. Rats in the sham operation group and the treatment groups received abdominal operation under anesthesia , and the gauze containing 0.9% normal saline, 0.05%, 0.1%, 0.25%, 0.5% benzalkonium chloride (BAC) was applied into colon for 30 minutes. Two weeks after operation, the number of feces, fecal dry weight in 24 h and gastrointestinal transit time were recorded, then hematoxylin-eosin (HE) staining, immunohistochemistry, ELISA were used for the evaluation of colonic pathology, enteric plexus, Interstitial cells of Cajal and neurotransmitters 5-hydroxytryptamine (5-HT). Results Compared to the normal control group and the sham operation group, the gastrointestinal transit time was significantly prolonged and fecal dry weight was lower in the treatment groupⅡ, Ⅲ (all P<0.05). HE and immunohistochemical staining showed varying degrees of pathological changes in the treatment groups and in line with the pathological changes of slow transit constipation. 5-HT concentration reduced significantly in treatment group Ⅲ compared to other groups (P<0.01). Conclusion The animal model of STC is successfully established by applying 0.25% BAC selective chemical ablation of the colon enteric plexus. This model is simple , stable, and is more in line with pathological changes of slow transit constipation.