中国糖尿病杂志
中國糖尿病雜誌
중국당뇨병잡지
CHINESE JOURNAL OF DIABETES
2015年
5期
452-455
,共4页
朱建华%郑承红%崔天盆%陈杰%黄炎
硃建華%鄭承紅%崔天盆%陳傑%黃炎
주건화%정승홍%최천분%진걸%황염
促生长激素释放肽%人脐静脉内皮细胞%哺乳类雷帕霉素靶蛋白%增殖
促生長激素釋放肽%人臍靜脈內皮細胞%哺乳類雷帕黴素靶蛋白%增殖
촉생장격소석방태%인제정맥내피세포%포유류뢰파매소파단백%증식
Ghrelin%Human umbilical vein endothelial cell (HUVEC)%mTOR%Proliferation
目的:探讨促生长激素释放肽(Ghrelin)促进人脐静脉内皮细胞(HUVEC)增殖的分子机制。方法将 HUVEC分为正常对照(Con)组、Ghr组、D‐Lys‐3‐GHRP组、Ghr+D‐Lys‐3‐GHRP组、雷帕霉素(Rapa)组及Ghr+Rapa组,采用MTT法检测细胞增殖能力,Western blot检测HUVEC中Gh‐relin受体(GHSR1a)的表达及哺乳类雷帕霉素靶蛋白(mTOR)、p70S6K和S6的磷酸化水平。结果与Con组相比,MTT检测显示Ghr组细胞增殖增强,且高于Ghr+D‐Lys‐3‐GHRP组和Ghr+ Rapa组;Western blot检测显示,与Con组比较,mTOR、p70S6K和S6在Ghr组快速磷酸化,Ghr+D‐Lys‐3‐GH‐RP组mTOR、P70S6K和S6磷酸化水平低于Ghr组。结论 Ghrelin与其受体GHSR1a结合后,活化mTOR/p70S6K信号通路,促进HUVEC增殖。
目的:探討促生長激素釋放肽(Ghrelin)促進人臍靜脈內皮細胞(HUVEC)增殖的分子機製。方法將 HUVEC分為正常對照(Con)組、Ghr組、D‐Lys‐3‐GHRP組、Ghr+D‐Lys‐3‐GHRP組、雷帕黴素(Rapa)組及Ghr+Rapa組,採用MTT法檢測細胞增殖能力,Western blot檢測HUVEC中Gh‐relin受體(GHSR1a)的錶達及哺乳類雷帕黴素靶蛋白(mTOR)、p70S6K和S6的燐痠化水平。結果與Con組相比,MTT檢測顯示Ghr組細胞增殖增彊,且高于Ghr+D‐Lys‐3‐GHRP組和Ghr+ Rapa組;Western blot檢測顯示,與Con組比較,mTOR、p70S6K和S6在Ghr組快速燐痠化,Ghr+D‐Lys‐3‐GH‐RP組mTOR、P70S6K和S6燐痠化水平低于Ghr組。結論 Ghrelin與其受體GHSR1a結閤後,活化mTOR/p70S6K信號通路,促進HUVEC增殖。
목적:탐토촉생장격소석방태(Ghrelin)촉진인제정맥내피세포(HUVEC)증식적분자궤제。방법장 HUVEC분위정상대조(Con)조、Ghr조、D‐Lys‐3‐GHRP조、Ghr+D‐Lys‐3‐GHRP조、뢰파매소(Rapa)조급Ghr+Rapa조,채용MTT법검측세포증식능력,Western blot검측HUVEC중Gh‐relin수체(GHSR1a)적표체급포유류뢰파매소파단백(mTOR)、p70S6K화S6적린산화수평。결과여Con조상비,MTT검측현시Ghr조세포증식증강,차고우Ghr+D‐Lys‐3‐GHRP조화Ghr+ Rapa조;Western blot검측현시,여Con조비교,mTOR、p70S6K화S6재Ghr조쾌속린산화,Ghr+D‐Lys‐3‐GH‐RP조mTOR、P70S6K화S6린산화수평저우Ghr조。결론 Ghrelin여기수체GHSR1a결합후,활화mTOR/p70S6K신호통로,촉진HUVEC증식。
Objective To investigate the molecular mechanism of ghrelin in promoting HUVECs proliferation. Methods HUVECs were divided into six groups :the normal control group ,the Ghr group ,the D‐Lys‐3‐GHRP group ,the Ghr+ D‐Lys‐3‐GHRP group ,the Rapa group and the Ghr+ Rapa group. The proliferation of HUVECs were determined by MTT assay. The expression of GHSR1a and the phosphorylation level of mTOR ,P70S6K and S6 were detected by western blot. Results Compared with the normal control group ,the proliferation of HUVEC was increased in ghrelin group ,and significantly higher in Ghr group than in Ghr+ D‐Lys‐3‐GHRP group and Ghr+ Rapa group. Western blot showed a rapid phosphorylation of mTOR ,P70S6K and S6 in Ghr group than in control group. The phosphorylation level of mTOR ,P70S6K and S6 in Ghr + D‐Lys‐3‐GHRP group was lower than in ghrelin group.Conclusion The results demonstrated that ghrelin promotes HUVECs proliferation by binding to GHSR1a and activating mTOR/P70S6K signaling pathway.