CAJ | 학술논문

目的：通过绝对定量分析健康青年人服用磷酸可待因后双侧前额叶及海马代谢物浓度变化，为下一步研究青年人可待因慢性成瘾机制提供前期实验数据参考。材料与方法正常健康青年志愿者20名，男10名，女10名，年龄18～30岁，平均(24.9±1.9)岁，均为右利手，无神经、精神疾病病史。采用 GE 1.5 T Signa HDX超导MR扫描仪，单体素1H-MRS PRESS序列，TR 3000 ms， TE 30 ms，矩阵256×128，NEX为1，感兴趣区置于双侧前额叶及双侧海马，大小2 cm×2 cm×2 cm，采集服药前及口服60 mg磷酸可待因后1.0～2 h内的实验数据，利用LCModel软件对采集的数据进行后处理及定量，用SPSS19.0配对样本t检验进行统计学分析。结果服用磷酸可待因后左前额叶脑代谢物发生改变，甘油磷酸胆碱(GPC)浓度增加0.254 mmol/L、肌醇(Ins)浓度下降0.988 mmol/L，差异有统计学意义(n＝13，P＜0.05；n＝12，P＜0.05)；右前额叶、双侧海马的代谢物浓度改变无统计学差异。结论口服磷酸可待因能引起左前额叶代谢物的改变，GPC浓度上升与Ins浓度下降可能与急性用药后前膜递质释放减少有关。
목적：통과절대정량분석건강청년인복용린산가대인후쌍측전액협급해마대사물농도변화，위하일보연구청년인가대인만성성은궤제제공전기실험수거삼고。재료여방법정상건강청년지원자20명，남10명，녀10명，년령18～30세，평균(24.9±1.9)세，균위우리수，무신경、정신질병병사。채용 GE 1.5 T Signa HDX초도MR소묘의，단체소1H-MRS PRESS서렬，TR 3000 ms， TE 30 ms，구진256×128，NEX위1，감흥취구치우쌍측전액협급쌍측해마，대소2 cm×2 cm×2 cm，채집복약전급구복60 mg린산가대인후1.0～2 h내적실험수거，이용LCModel연건대채집적수거진행후처리급정량，용SPSS19.0배대양본t검험진행통계학분석。결과복용린산가대인후좌전액협뇌대사물발생개변，감유린산담감(GPC)농도증가0.254 mmol/L、기순(Ins)농도하강0.988 mmol/L，차이유통계학의의(n＝13，P＜0.05；n＝12，P＜0.05)；우전액협、쌍측해마적대사물농도개변무통계학차이。결론구복린산가대인능인기좌전액협대사물적개변，GPC농도상승여Ins농도하강가능여급성용약후전막체질석방감소유관。
Objective: Absolute quantitative analysis of healthy youth in the bilateral prefrontal lobes and hippocampus were performed and offered preliminary stage experimental data, in order to supply a reference for the further study of codeine habituation.Materials and Methods: Twenty right-handed healthy youth were included [10 males, 10 females, mean age (24.9±1.9) years]. MRS data were collected by GE 1.5 T MR scanner, using point resolved selective spectroscopy (PRESS) sequence (TE/TR 30 ms/3000 ms). The regions of interest (ROI) were located over the bilateral prefrontal lobes and hippocampus, size of ROI was 2 cm × 2 cm × 2 cm. The data of spectra were post-processed by LCModel software and the concentrations of metabolites were ultimately measured. The metabolite concentration of each people was analyzed by paired-samples ttest of SPSS19.0.Results:After taking codeine phosphate, the concentration of GPC increased 0.254 mmol/L in the left prefrontal lobe while Ins declined 0.988 mmol/L, which reached statistical signiifcance (n=13,P<0.05. n=12,P<0.05). The alterations of metabolites in right prefrontal lobe and bilateral hippocampus did not reach statistical significance.Conclusions:The metabolites’ concentrations of the left prefrontal lobes would change with oral codeine phosphate. GPC increased in the left prefrontal lobe while Ins declined, which was considered related with the abatement of neurotransmitter.