CAJ | 학술논문

目的：探讨关于鼠肾小管上皮细胞原代培养的有效方法。方法在现有肾小管上皮细胞机械研磨方法的基础上，通过SD大鼠与昆明小鼠的对比实验，Ⅰ型胶原酶消化时间的设定以及第一次换取液的合理利用等方面改进原代培养的方法。对改进后所分离的肾小管上皮细胞进行鉴定。结果与SD大鼠相比，昆明小鼠细胞贴壁生长多且快，胶原蛋消化25分钟后的细胞贴壁多、长满壁底早。将第一次细胞换取液进行再次贴壁，第二天依旧可以有部分细胞贴壁，3~4天长满壁底，纯度较高。两次细胞均成上皮细胞鹅卵石铺路样生长，经免疫细胞化学法（SABC法）鉴定CK表达阳性。结论实验选择昆明小鼠肾小管上皮细胞经胶原酶消化25分钟左右的细胞数量、质量较好，第一次细胞废弃液可以再次有效利用。
목적：탐토관우서신소관상피세포원대배양적유효방법。방법재현유신소관상피세포궤계연마방법적기출상，통과SD대서여곤명소서적대비실험，Ⅰ형효원매소화시간적설정이급제일차환취액적합리이용등방면개진원대배양적방법。대개진후소분리적신소관상피세포진행감정。결과여SD대서상비，곤명소서세포첩벽생장다차쾌，효원단소화25분종후적세포첩벽다、장만벽저조。장제일차세포환취액진행재차첩벽，제이천의구가이유부분세포첩벽，3~4천장만벽저，순도교고。량차세포균성상피세포아란석포로양생장，경면역세포화학법（SABC법）감정CK표체양성。결론실험선택곤명소서신소관상피세포경효원매소화25분종좌우적세포수량、질량교호，제일차세포폐기액가이재차유효이용。
Objective:To explore the effective methods for the primary culture of rat renal tubular epithelial cells. Methods:On the basis of the mechanical grinding for the obtainment of tubular epithelial cells, we compared the difference between the experimental SD rats and KunMing mice as the source and optimized theⅠcollagenase digestion time as well as the rational use of liquid that was quitted for the ifrst time. The identiifcation of the collected renal tubular epithelial cells was performed by immunocytochemistry. Results:Compared with SD rats, renal tubular epithelial cells isolated from KunMing mice presented better growth rate with 25 minutes’ Ⅰcollagenase digestion. Additionally, the amount and quality of cells which recovered from the ifrst cell waste liquid were good, showing an effective reutilization. Conclusion:Mechanical grinding and 25 minutes’Ⅰcollagenase digestion with KunMing mice as source is an ideal method for the primary culture of the renal tubular epithelial cells. And the ifrst cell waste liquid could be reused well.