世界中医药
世界中醫藥
세계중의약
WORLD CHINESE MEDICINE
2015年
5期
757-761
,共5页
郝改梅%何洁%韩静%吴晏%武志黔%袁悦莹%毛颖秋%王伟%毕力夫
郝改梅%何潔%韓靜%吳晏%武誌黔%袁悅瑩%毛穎鞦%王偉%畢力伕
학개매%하길%한정%오안%무지검%원열형%모영추%왕위%필력부
糖尿病视网膜病变%活血解毒方%血管内皮生长因子%Ras/Raf-1/ERK信号转导通路
糖尿病視網膜病變%活血解毒方%血管內皮生長因子%Ras/Raf-1/ERK信號轉導通路
당뇨병시망막병변%활혈해독방%혈관내피생장인자%Ras/Raf-1/ERK신호전도통로
Diabetic retinopathy ( DR )%Huoxue Jiedu recipe ( HJR )%Vascular endothelial growth factor ( VEGF )%Ras/Raf-1/ERK signal transduction pathway
目的:观察活血解毒方对糖尿病大鼠视网膜血管形态的影响并探讨其可能的作用机制。方法:一次性腹腔注射链脲佐菌素(Streptozotocin,STZ,65 mg/kg)诱导糖尿病大鼠模型,造模后随机分为模型组和活血解毒方组。另设正常对照组。药物干预12周后,应用视网膜消化铺片法观察视网膜血管形态;应用Western blot法检测视网膜VEGF蛋白的表达,应用Real-Time PCR法检测视网膜VEGF、Ras、Raf-1与ERK mRNA的表达。结果:模型组视网膜毛细血管面积密度及内皮细胞/周细胞比例与较正常组升高(P<0.001),VEGF蛋白及VEGF、Ras、ERKmRNA表达升高(P<0.05),Raf-1mRNA表达升高( P>0.05)。与模型组比较,活血解毒方组视网膜毛细血管面积密度和内皮细胞/周细胞比例降低( P<0.01和P<0.001),VEGF蛋白与VEGF、Ras、Raf-1、ERK mRNA表达下降( P<0.05)。结论:活血解毒方能明显抑制视网膜毛细血管和内皮细胞的增生,其机制可能是通过干预Ras/Raf-1/ERK信号转导通路,下调VEGF在视网膜中的表达,抑制血管新生,从而改善DR。
目的:觀察活血解毒方對糖尿病大鼠視網膜血管形態的影響併探討其可能的作用機製。方法:一次性腹腔註射鏈脲佐菌素(Streptozotocin,STZ,65 mg/kg)誘導糖尿病大鼠模型,造模後隨機分為模型組和活血解毒方組。另設正常對照組。藥物榦預12週後,應用視網膜消化鋪片法觀察視網膜血管形態;應用Western blot法檢測視網膜VEGF蛋白的錶達,應用Real-Time PCR法檢測視網膜VEGF、Ras、Raf-1與ERK mRNA的錶達。結果:模型組視網膜毛細血管麵積密度及內皮細胞/週細胞比例與較正常組升高(P<0.001),VEGF蛋白及VEGF、Ras、ERKmRNA錶達升高(P<0.05),Raf-1mRNA錶達升高( P>0.05)。與模型組比較,活血解毒方組視網膜毛細血管麵積密度和內皮細胞/週細胞比例降低( P<0.01和P<0.001),VEGF蛋白與VEGF、Ras、Raf-1、ERK mRNA錶達下降( P<0.05)。結論:活血解毒方能明顯抑製視網膜毛細血管和內皮細胞的增生,其機製可能是通過榦預Ras/Raf-1/ERK信號轉導通路,下調VEGF在視網膜中的錶達,抑製血管新生,從而改善DR。
목적:관찰활혈해독방대당뇨병대서시망막혈관형태적영향병탐토기가능적작용궤제。방법:일차성복강주사련뇨좌균소(Streptozotocin,STZ,65 mg/kg)유도당뇨병대서모형,조모후수궤분위모형조화활혈해독방조。령설정상대조조。약물간예12주후,응용시망막소화포편법관찰시망막혈관형태;응용Western blot법검측시망막VEGF단백적표체,응용Real-Time PCR법검측시망막VEGF、Ras、Raf-1여ERK mRNA적표체。결과:모형조시망막모세혈관면적밀도급내피세포/주세포비례여교정상조승고(P<0.001),VEGF단백급VEGF、Ras、ERKmRNA표체승고(P<0.05),Raf-1mRNA표체승고( P>0.05)。여모형조비교,활혈해독방조시망막모세혈관면적밀도화내피세포/주세포비례강저( P<0.01화P<0.001),VEGF단백여VEGF、Ras、Raf-1、ERK mRNA표체하강( P<0.05)。결론:활혈해독방능명현억제시망막모세혈관화내피세포적증생,기궤제가능시통과간예Ras/Raf-1/ERK신호전도통로,하조VEGF재시망막중적표체,억제혈관신생,종이개선DR。
Objective:To observe the effect of Huoxue Jiedu Recipe ( HJR) on the morphology of retinal blood vessels of diabetic rats, and to explore its underlying mechanism .Methods:Streptozotocin(STZ, 65 mg/kg) was intraperitoneally injected to rats to induce diabetes .The diabetic rats were randomly divided into the model group and the HJR group .Other rats were recruited as the normal control group .After 12 weeks intervention ,the retinal vascular morphology was observed by using trypsin digestion .The ex-pression of VEGF protein was examined by Western-blot.The expression of VEGF, Ras, Raf-1, ERK mRNA in retinas of rats was examined by Real-time PCR.Results:Compared with the normal group , capillary density and the ratio of endothelial cells to peri-cytes increased in the model group ( P<0.001 ) .VEGF protein and VEGF mRNA , Ras mRNA, ERK mRNA expression increased ( P<0.05 ) , Raf-1 mRNA expression increased ( P>0.05 ) .Compared with the model group , capillary density decreased in the HJR group ( P<0.01 ) , and the ratio of endothelial cells to pericytes decreased ( P<0.001 ) .VEGF protein and VEGF mRNA , Ras mRNA, Raf-1 mRNA, ERK mRNA expression decreased(P<0.05).Conclusion:HJR could obviously restrain the prolifera-tion of capillary and endothelial cells .The effect is mediated by down-regulating expression of VEGF which is achieved by inhibi-ting Ras/Raf-1/ERK signaling pathway .