CAJ | 학술논문

目的：建立一种简单易行，快速，准确的母血污染鉴定技术，应用于产前诊断前排除母血污染。方法在 X染色体上选取具有高杂合度和高多态性的6个短串联重复序列位点，应用连锁分析对10例羊水和10例脐带血标本进行母血污染鉴定。结果在10例羊水和10例脐带血标本中各发现1例标本发生母血污染。结论该方法可应用于临床实验室进行产前诊断前排除母血污染。
목적：건립일충간단역행，쾌속，준학적모혈오염감정기술，응용우산전진단전배제모혈오염。방법재 X염색체상선취구유고잡합도화고다태성적6개단천련중복서렬위점，응용련쇄분석대10례양수화10례제대혈표본진행모혈오염감정。결과재10례양수화10례제대혈표본중각발현1례표본발생모혈오염。결론해방법가응용우림상실험실진행산전진단전배제모혈오염。
Objective To establish a simple,rapid,accurate maternal blood contamination identification technology, used to exclude maternal blood contamination before prenatal diagnosis.Methods 6 short tandem repeat loci on the X chromosome with high heterozygosity and polymorphism were selected to identify maternal blood contamination from 10 cases of amniotic fluid and 10 cases of umbilical cord blood specimens by linkageanalysis.Results In 20 cases of samples,one case of amniotic fluid and one case of umbilical cord blood samples occurred in maternal blood contamina-tion,respectively.And the experiment results agree with expected results.Conclusion This method can be used to ex-clude maternal blood contamination before prenatal diagnosis in clinical laboratories.