CAJ | 학술논문

目的：比较裸鼠腋下皮肤切开缝合和穿刺套针注射接种人鼻咽癌细胞成瘤效果。方法制备鼻咽癌HONE-1细胞裸鼠皮下移植瘤块样本。将8只BALB／c裸鼠随机分为A、B组，各4只。 A、B组分别使用皮肤切开缝合接种和穿刺套针接种方法于裸鼠右侧腋下接种细胞株移植瘤组织块，比较两组裸鼠肿瘤出现时间、体积、质量。结果 A、B组肿瘤出现时间均为接种后第3天。与B组相比，A组裸鼠较早出现精神萎靡、皮肤粗糙、毛发光泽不良、食欲不振、活动量减低、脊椎明显、弓背等状况。两组裸鼠体质量均有所下降，A组比B组肿瘤生长迅速，但差异无统计学意义。 A、B组肿瘤质量分别为（0．85±0．39）、（0．75±0．30） g，两组比较，P＞0．05。结论裸鼠腋下皮肤切开缝合和穿刺套针注射接种人鼻咽癌细胞均能达到好的成瘤效果。
목적：비교라서액하피부절개봉합화천자투침주사접충인비인암세포성류효과。방법제비비인암HONE-1세포라서피하이식류괴양본。장8지BALB／c라서수궤분위A、B조，각4지。 A、B조분별사용피부절개봉합접충화천자투침접충방법우라서우측액하접충세포주이식류조직괴，비교량조라서종류출현시간、체적、질량。결과 A、B조종류출현시간균위접충후제3천。여B조상비，A조라서교조출현정신위미、피부조조、모발광택불량、식욕불진、활동량감저、척추명현、궁배등상황。량조라서체질량균유소하강，A조비B조종류생장신속，단차이무통계학의의。 A、B조종류질량분별위（0．85±0．39）、（0．75±0．30） g，량조비교，P＞0．05。결론라서액하피부절개봉합화천자투침주사접충인비인암세포균능체도호적성류효과。
Objective To compare the tumorigenic effect between the inoculating method of slicing the subaxillary skin and suturing and the method of adopting the paracentesis trocar of human nasopharyngeal carcinoma on the nude mice. Methods The samples of subcutaneous transplanted tumors in nude mice were established with nasopharyngeal carcinoma HONE-1 cells.Eight BALB/c nude mice were randomly divided into two groups:group A and B, 4 mice in each group. The right subaxillary skin of each mouse was subcutaneously inoculated with the tissue masses through slicing the skin, or adopting the paracentesis trocar in the two groups, respectively.The occurrence time, volume and mass of tumor in each group were compared.Results The occurrence time of tumor in each group was 3 days after being inoculated.Compared with group B, the occurrence time of dispirited mind, coarse skin, shineless hair, loss of appetite, low activity, distinct spinal column, hunched backs and other conditions were earlier in the group A.The weight of mice was decreased in both groups, the growth of tumor in the group A was faster than that of the group B, but no significant difference was found be-tween these two groups.The mass of tumor in the group A and group B was (0.85 ±0.39) g and (0.75 ±0.30) g, re-spectively;and no significant difference was found between these two groups (P>0.05).Conclusion Both the inocula-ting method of slicing the subaxillary skin and suturing and the method of adopting the paracentesis trocar of human naso-pharyngeal carcinoma on the nude mice could receive finely tumorigenic effect.