CAJ | 학술논문

目的：探讨血管紧张素Ⅱ受体拮抗剂厄贝沙坦对糖尿病大鼠肾脏 chemerin 的影响。方法将 SD 大鼠随机分为对照组、糖尿病组和厄贝沙坦治疗组，以链脲佐菌素（STZ）及高糖高脂饮食制备2型糖尿病大鼠模型，给予厄贝沙坦40 mg／（kg·d）治疗8周后处死大鼠。Western blot 检测各组肾组织 chemerin 蛋白的表达。应用放射免疫法检测血清血管紧张素Ⅱ（AngⅡ）的含量。结果与对照组相比，糖尿病组血肌酐、尿素氮、24小时尿白蛋白排泄率及AngⅡ水平均显著升高（P ＜0．05），厄贝沙坦治疗后血肌酐及24小时尿白蛋白排泄率较糖尿病组显著降低（P ＜0.05）。糖尿病组大鼠肾组织中 chemerin 蛋白表达明显高于对照组，治疗组表达水平明显下降。结论 chemerin 在糖尿病大鼠肾脏中表达增加，厄贝沙坦可能通过抑制 RAS 系统降低 chemerin 的表达，从而对肾脏起保护作用。
목적：탐토혈관긴장소Ⅱ수체길항제액패사탄대당뇨병대서신장 chemerin 적영향。방법장 SD 대서수궤분위대조조、당뇨병조화액패사탄치료조，이련뇨좌균소（STZ）급고당고지음식제비2형당뇨병대서모형，급여액패사탄40 mg／（kg·d）치료8주후처사대서。Western blot 검측각조신조직 chemerin 단백적표체。응용방사면역법검측혈청혈관긴장소Ⅱ（AngⅡ）적함량。결과여대조조상비，당뇨병조혈기항、뇨소담、24소시뇨백단백배설솔급AngⅡ수평균현저승고（P ＜0．05），액패사탄치료후혈기항급24소시뇨백단백배설솔교당뇨병조현저강저（P ＜0.05）。당뇨병조대서신조직중 chemerin 단백표체명현고우대조조，치료조표체수평명현하강。결론 chemerin 재당뇨병대서신장중표체증가，액패사탄가능통과억제 RAS 계통강저 chemerin 적표체，종이대신장기보호작용。
Objective To study the effects of renin-angiotensin system (RAS)on the expression of chemerin in streptozotocin-induced diabetic rats.Methods After 6-week feeding with high-fat and high-carbohydrate diet,rats were administrated with STZ and randomized to control,diabetic,irbesartan-treated groups.The chemerin protein was identi-fied by western blot.The level of angiotensin II (AngⅡ )was measured by radioimmunoassay.Results Blood urea ni-trogen (BUN),serum creatinine (Scr),urinary albumin excretion rate(UAER)and AngⅡ levels in diabetic group were much higher than that in the control group.The level of chemerin in the renal tissues was significantly elevated in the diabetic group compared with the control group.Eight weeks after Irbesartan treatment,the Scr,UAER and chemerin were significant decreased.Conclusion Irbesartan treatment ameliorate renal function through an inhibitory effect on the activation of intrarenal RAS,which may impact chemerin expression levels in the kidney of diabetic rats.