实用医学杂志
實用醫學雜誌
실용의학잡지
THE JOURNAL OF PRACTICAL MEDICINE
2015年
9期
1434-1438
,共5页
耿宁%姜曼%王健%刘洋%姜相君%辛永宁
耿寧%薑曼%王健%劉洋%薑相君%辛永寧
경저%강만%왕건%류양%강상군%신영저
脂肪肝, 非酒精性%单核苷酸多态性%PARVB基因
脂肪肝, 非酒精性%單覈苷痠多態性%PARVB基因
지방간, 비주정성%단핵감산다태성%PARVB기인
Fatty liver%Non-alcoholic fatty liver disease%Gene polymorphism%PARVB
目的：探讨小细胞蛋白β基因（PARVB基因）rs5764455多态性与青岛汉族人群非酒精性脂肪性肝病（NAFLD）遗传易感性的相关性。方法：收集青岛地区B 型超声诊断为 NAFLD 的患者（NAFLD 组，n ＝230）及排除肝脏相关疾病的健康人群（对照组，n ＝330）。多聚酶链反应及DNA测序法进行PARVB基因型测定。检测并比较各组人群临床基本信息差异有无统计学意义。计算各组基因型频率、等位基因频率分布及发生NAFLD相对风险。结果：PARVB基因rs5764455位点基因型频率及等位基因频率在NAFLD组与对照组中的分布差异无统计学意义（基因型频率χ2＝0．182，P ＝0．913；等位基因频率χ2＝0．180，P ＝0．672）。校正体质量、BMI 等混杂因素前后，C／T ＋ T／T 基因型携带者与C／C 基因型携带者相比，发生 NAFLD 的风险度无统计学差异（OR ＝1．266，P ＝0．178；校正OR ＝1．631，P ＝0．096）。与C／C 基因型携带者相比，C／T＋ T／T 基因型携带者体质量、BMI、甘油三酯及ALT、AST 升高且差异有统计学意义（P ＜0．05）。结论：在青岛汉族人群中，PARVB 基因rs5764455多态性与NAFLD不相关。
目的：探討小細胞蛋白β基因（PARVB基因）rs5764455多態性與青島漢族人群非酒精性脂肪性肝病（NAFLD）遺傳易感性的相關性。方法：收集青島地區B 型超聲診斷為 NAFLD 的患者（NAFLD 組，n ＝230）及排除肝髒相關疾病的健康人群（對照組，n ＝330）。多聚酶鏈反應及DNA測序法進行PARVB基因型測定。檢測併比較各組人群臨床基本信息差異有無統計學意義。計算各組基因型頻率、等位基因頻率分佈及髮生NAFLD相對風險。結果：PARVB基因rs5764455位點基因型頻率及等位基因頻率在NAFLD組與對照組中的分佈差異無統計學意義（基因型頻率χ2＝0．182，P ＝0．913；等位基因頻率χ2＝0．180，P ＝0．672）。校正體質量、BMI 等混雜因素前後，C／T ＋ T／T 基因型攜帶者與C／C 基因型攜帶者相比，髮生 NAFLD 的風險度無統計學差異（OR ＝1．266，P ＝0．178；校正OR ＝1．631，P ＝0．096）。與C／C 基因型攜帶者相比，C／T＋ T／T 基因型攜帶者體質量、BMI、甘油三酯及ALT、AST 升高且差異有統計學意義（P ＜0．05）。結論：在青島漢族人群中，PARVB 基因rs5764455多態性與NAFLD不相關。
목적：탐토소세포단백β기인（PARVB기인）rs5764455다태성여청도한족인군비주정성지방성간병（NAFLD）유전역감성적상관성。방법：수집청도지구B 형초성진단위 NAFLD 적환자（NAFLD 조，n ＝230）급배제간장상관질병적건강인군（대조조，n ＝330）。다취매련반응급DNA측서법진행PARVB기인형측정。검측병비교각조인군림상기본신식차이유무통계학의의。계산각조기인형빈솔、등위기인빈솔분포급발생NAFLD상대풍험。결과：PARVB기인rs5764455위점기인형빈솔급등위기인빈솔재NAFLD조여대조조중적분포차이무통계학의의（기인형빈솔χ2＝0．182，P ＝0．913；등위기인빈솔χ2＝0．180，P ＝0．672）。교정체질량、BMI 등혼잡인소전후，C／T ＋ T／T 기인형휴대자여C／C 기인형휴대자상비，발생 NAFLD 적풍험도무통계학차이（OR ＝1．266，P ＝0．178；교정OR ＝1．631，P ＝0．096）。여C／C 기인형휴대자상비，C／T＋ T／T 기인형휴대자체질량、BMI、감유삼지급ALT、AST 승고차차이유통계학의의（P ＜0．05）。결론：재청도한족인군중，PARVB 기인rs5764455다태성여NAFLD불상관。
Objective To investigate the association between (beta-parvin) PARVB gene rs5764455 polymorphism and susceptibility to non-alcoholic fatty liver disease (NAFLD). Methods A total of 230 patients with NAFLD (NAFLD, n = 230) and 230 control subjects (control, n = 330) were genotyped by PCR and direct sequencing. Clinical information was detected and compared in different groups. Genotypic frequency and gene frequency distribution in the two groups and relative risks to NAFLD susceptibility were assessed statistically , respectively. Results No statistical differences were observed between PARVB gene rs5764455 genotypic frequency with gene frequency distribution and the two groups, respectively (Genotypic frequency χ2 = 0.182, P = 0.913; gene frequency χ2 = 0.180, P = 0.672). Comparing C/T + T/T genotype carrier with C/C genotype carrier, there were no differences concerning the relative risks to NAFLD susceptibility (OR = 1.266, P =0.178;adjusted OR =1.631, P =0.096) before and after adjusting body mass, BMI and so on. In the latter group, there are significant differences in the increases of body mass, BMI, TG, ALT and AST (P < 0.05). Conclusion Non-relationship was observed between PARVB gene rs5764455 polymorphism and the risk of NAFLD in Qingdao Han Chinese.
