CAJ | 학술논문

目的：观察痛泻要方对腹泻型肠易激综合征（ D－IBS）实验大鼠结肠运动指数（每分钟收缩强度曲线下面积，MI）和收缩频率（每分钟收缩次数，CF）的影响。方法：将SPF级新生SD雄性大鼠随机分为正常组、模型组、中药痛泻要方组。除正常组以外，其他2组均采用母婴分离加束缚应激的方法进行造模。第60天造模成功后，于第61天开始，正常组和模型组给予生理盐水，中药组给予4．92 g／100 g痛泻要方煎剂。3组均采用灌胃的方法给药，给药体积均为2 mL／（100 g?d），连续14 d。造模及给药过程中观察大鼠粪便Bristol分级评分及粪便含水量。采用离体张力测定灌流系统，测量3组大鼠离体结肠纵型平滑肌肌条（CLSMs）运动指数（MI，mg／min）和收缩频率（CF，times／min）的变化。结果：给药前，中药组和模型组粪便Bristol评分、含水量均高于正常组（ P＜0．05）；给药后，中药组粪便Bristol评分及含水量均低于模型组（ P＜0．01）。给药前，中药组和模型组的运动指数（MI）、收缩频率（CF）结果近似，且均高于正常组（P＜0．01）；给药后，中药组的MI、CF均呈下降趋势，且低于模型组（ P＜0．01），与正常组比较无统计学意义（ P＞0．05）。结论：痛泻要方可有效改善D－IBS大鼠的腹泻症状，其作用机制可能是通过降低结肠平滑肌的运动指数及收缩频率实现的。
목적：관찰통사요방대복사형장역격종합정（ D－IBS）실험대서결장운동지수（매분종수축강도곡선하면적，MI）화수축빈솔（매분종수축차수，CF）적영향。방법：장SPF급신생SD웅성대서수궤분위정상조、모형조、중약통사요방조。제정상조이외，기타2조균채용모영분리가속박응격적방법진행조모。제60천조모성공후，우제61천개시，정상조화모형조급여생리염수，중약조급여4．92 g／100 g통사요방전제。3조균채용관위적방법급약，급약체적균위2 mL／（100 g?d），련속14 d。조모급급약과정중관찰대서분편Bristol분급평분급분편함수량。채용리체장력측정관류계통，측량3조대서리체결장종형평활기기조（CLSMs）운동지수（MI，mg／min）화수축빈솔（CF，times／min）적변화。결과：급약전，중약조화모형조분편Bristol평분、함수량균고우정상조（ P＜0．05）；급약후，중약조분편Bristol평분급함수량균저우모형조（ P＜0．01）。급약전，중약조화모형조적운동지수（MI）、수축빈솔（CF）결과근사，차균고우정상조（P＜0．01）；급약후，중약조적MI、CF균정하강추세，차저우모형조（ P＜0．01），여정상조비교무통계학의의（ P＞0．05）。결론：통사요방가유효개선D－IBS대서적복사증상，기작용궤제가능시통과강저결장평활기적운동지수급수축빈솔실현적。
Objective:To observe the effects of Tong-Xie-Yao-Fang ( TXYF) formula on movement index ( MI, area under the curve of contraction intensity/min) and contraction frequency ( CF, number of contractions/min) of experimental rats with diarrhe-a-predominant irritable bowel syndrome ( D-IBS ) .Methods: The newborn Specific Pathogen Free ( SPF ) and Sprague-Dawley ( SD) male pups were randomly divided into normal group , NMS+RS group and TXYF formula group .In addition to the normal group, D-IBS model with the method of neonatal maternal separation plus restraint stress ( NMS+RS) were established in other two groups.On the 61st day, after the success of molding , normal group and NMS +RS group was given with saline , while TXYF formula group were given with 4.92 g/100 g TXYF formula.Three groups were dosed with the method of gavage with the drug de-livery volume of 2 mL/100 g/d, for 14 consecutive days .The scores of Bristol classification and water content of the fecal pellets of rats were observed in the process of model building and dosing .The distinctions of movement index (MI, area under the curve of contraction intensity/min, mg/min) and contraction frequency ( CF, number of contractions/min, times/min) of isolated colon-ic longitudinal smooth muscle strips (CLSMs) in three groups before and after treatment were observed with a Power Lab System . Results:Before treatment , scores of Bristol classification and water content of the fecal pellets in both TXYF formula group and NMS+RS group were all higher than that in normal group (P<0.05);After treatment, the scores of Bristol classification and wa-ter content of the fecal pellets in TXYF formula group were lower than that in NMS +RS group ( P<0.01 ) .Before treatment , the MI and CF of CLSMs in the NMS +RS and TXYF formula groups were similar and all higher than that in the normal group ( P<0.01, respectively).After treatment, the MI and CF in the TXYF formula group was lower than in the NMS +RS group (P<0.01) and similar to the normal group (P>0.05).Conclusion:TXYF formula can effectively improve symptoms of D-IBS rats. Its mechanism may be achieved through Lower colon movement index and contraction frequency .