世界中医药
世界中醫藥
세계중의약
WORLD CHINESE MEDICINE
2015年
5期
664-670
,共7页
何宜航%杨桂燕%阳向波%邹俊波%纪奇森%张帅杰%杨明
何宜航%楊桂燕%暘嚮波%鄒俊波%紀奇森%張帥傑%楊明
하의항%양계연%양향파%추준파%기기삼%장수걸%양명
熟三七%Lewis肺癌%抑制%联合用药%增效减毒
熟三七%Lewis肺癌%抑製%聯閤用藥%增效減毒
숙삼칠%Lewis폐암%억제%연합용약%증효감독
Steamed Panax notoginseng%Lewis lung cancer%Tumor inhibition%Combination%Effect-enhancing and toxicity-re-ducing effect
目的：考察熟三七粉对Lewis肺癌的抑制作用及与紫杉醇、培美曲塞二钠联合用药的增效减毒作用。方法：首先将接种Lewis肺癌的C57 BL／6小鼠按体重随机分为模型组，阳性组，生、熟三七低、中、高剂量组，每组13～14只。其中生、熟三七粉按低、中、高剂量（585 mg? kg－1，1170 mg? kg－1，2340 mg? kg－1）连续灌胃15 d，阳性组以60 mg? kg －1剂量于第1天腹腔注射CTX 1次，试验结束后计算抑瘤率，并统计给药前后体重、体重比值（ FBW／IBW）、死亡情况；再将新接种Lewis肺癌的C57BL／6小鼠按体重随机分为9组，每组10只，即模型组（0．5％ CMC－Na溶液）、熟三七组、生三七组、紫杉醇组、熟三七联合紫杉醇组、生三七联合紫杉醇组、ALIMTA组、熟三七联合ALIMTA组、生三七联合ALIMTA组。生、熟三七以1170 mg? kg－1连续灌胃给药15 d，紫杉醇（10 mg? kg－1）、ALIMTA（120．3 mg? kg－1）自给药第1天起每3 d腹腔注射1次，共3次。试验结束后计算抑瘤率，测定外周血象，并考察给药前后体重、体重比值（ FBW／IBW）、死亡情况。结果：与模型组相比，熟三七（1170 mg? kg－1）具有极显著抑瘤率41％（P＜0．01）、生三七（2340 mg? kg－1）具有显著抑瘤率（P＜0．05），但无临床意义，且表现出一定不良反应；生、熟三七与紫杉醇、ALIMTA连用均能提高抑瘤率，且熟三七表现出降低紫杉醇毒副作用的趋势，而生三七却表现出一定不良反应。结论：熟三七能显著抑制Lewis肺癌生长，与化药联合使用可表现出较好协同作业用，并可拮抗紫杉醇毒副作用；生三七对肿瘤生长的抑制不具有临床意义，虽在联合用药中表现出较好的协同作用，但不良反应明显。
目的：攷察熟三七粉對Lewis肺癌的抑製作用及與紫杉醇、培美麯塞二鈉聯閤用藥的增效減毒作用。方法：首先將接種Lewis肺癌的C57 BL／6小鼠按體重隨機分為模型組，暘性組，生、熟三七低、中、高劑量組，每組13～14隻。其中生、熟三七粉按低、中、高劑量（585 mg? kg－1，1170 mg? kg－1，2340 mg? kg－1）連續灌胃15 d，暘性組以60 mg? kg －1劑量于第1天腹腔註射CTX 1次，試驗結束後計算抑瘤率，併統計給藥前後體重、體重比值（ FBW／IBW）、死亡情況；再將新接種Lewis肺癌的C57BL／6小鼠按體重隨機分為9組，每組10隻，即模型組（0．5％ CMC－Na溶液）、熟三七組、生三七組、紫杉醇組、熟三七聯閤紫杉醇組、生三七聯閤紫杉醇組、ALIMTA組、熟三七聯閤ALIMTA組、生三七聯閤ALIMTA組。生、熟三七以1170 mg? kg－1連續灌胃給藥15 d，紫杉醇（10 mg? kg－1）、ALIMTA（120．3 mg? kg－1）自給藥第1天起每3 d腹腔註射1次，共3次。試驗結束後計算抑瘤率，測定外週血象，併攷察給藥前後體重、體重比值（ FBW／IBW）、死亡情況。結果：與模型組相比，熟三七（1170 mg? kg－1）具有極顯著抑瘤率41％（P＜0．01）、生三七（2340 mg? kg－1）具有顯著抑瘤率（P＜0．05），但無臨床意義，且錶現齣一定不良反應；生、熟三七與紫杉醇、ALIMTA連用均能提高抑瘤率，且熟三七錶現齣降低紫杉醇毒副作用的趨勢，而生三七卻錶現齣一定不良反應。結論：熟三七能顯著抑製Lewis肺癌生長，與化藥聯閤使用可錶現齣較好協同作業用，併可拮抗紫杉醇毒副作用；生三七對腫瘤生長的抑製不具有臨床意義，雖在聯閤用藥中錶現齣較好的協同作用，但不良反應明顯。
목적：고찰숙삼칠분대Lewis폐암적억제작용급여자삼순、배미곡새이납연합용약적증효감독작용。방법：수선장접충Lewis폐암적C57 BL／6소서안체중수궤분위모형조，양성조，생、숙삼칠저、중、고제량조，매조13～14지。기중생、숙삼칠분안저、중、고제량（585 mg? kg－1，1170 mg? kg－1，2340 mg? kg－1）련속관위15 d，양성조이60 mg? kg －1제량우제1천복강주사CTX 1차，시험결속후계산억류솔，병통계급약전후체중、체중비치（ FBW／IBW）、사망정황；재장신접충Lewis폐암적C57BL／6소서안체중수궤분위9조，매조10지，즉모형조（0．5％ CMC－Na용액）、숙삼칠조、생삼칠조、자삼순조、숙삼칠연합자삼순조、생삼칠연합자삼순조、ALIMTA조、숙삼칠연합ALIMTA조、생삼칠연합ALIMTA조。생、숙삼칠이1170 mg? kg－1련속관위급약15 d，자삼순（10 mg? kg－1）、ALIMTA（120．3 mg? kg－1）자급약제1천기매3 d복강주사1차，공3차。시험결속후계산억류솔，측정외주혈상，병고찰급약전후체중、체중비치（ FBW／IBW）、사망정황。결과：여모형조상비，숙삼칠（1170 mg? kg－1）구유겁현저억류솔41％（P＜0．01）、생삼칠（2340 mg? kg－1）구유현저억류솔（P＜0．05），단무림상의의，차표현출일정불량반응；생、숙삼칠여자삼순、ALIMTA련용균능제고억류솔，차숙삼칠표현출강저자삼순독부작용적추세，이생삼칠각표현출일정불량반응。결론：숙삼칠능현저억제Lewis폐암생장，여화약연합사용가표현출교호협동작업용，병가길항자삼순독부작용；생삼칠대종류생장적억제불구유림상의의，수재연합용약중표현출교호적협동작용，단불량반응명현。
Objective:To Investigate the inhibition effect of steamed Panax notoginseng on Lewis lung cancer , and find out wheth-er there is a effect-enhancing and toxicity-reducing effect when use in combination with paclitaxel or pemetrexed disodium .Meth-ods:C57 BL/6 mice inoculated with Lewis lung cancer were randomly divided into model group , positive group , raw Panax notog-inseng low, medium and high dose group , steamed Panax notoginseng low , medium and high dose group ( each group n =13~14).Among them, raw and steamed Panax notoginseng groups received intragastric administration according to low , middle, high dose(585 mg? kg -1, 1170 mg? kg-1, 2340 mg? kg-1), positive group mice received intraperitoneal injection of CTX (60 mg?kg -1 ) on the first day.After the trial, calculated inhibition rate against tumor and recorded body weight before /after administra-tion, body weight ratio(FBW/IBW) and death.Then the new Lewis lung cancer inoculated C57BL/6 mice were randomly divided into 9 groups (n =10), model group (0.5% CMC-Na solution), steamed Panax notoginseng group, raw Panax notoginseng group, paclitaxel group, steamed Panax notoginseng plus paclitaxel group , raw Panax notoginseng plus paclitaxel group , ALIMTA group, steamed Panax notoginseng plus ALIMTA group and raw Panax notoginseng plus ALIMTA group .Steamed and raw Panax notoginseng were continuously intragastric administrated at the dose of 1170 mg? kg -1, and paclitaxel(15 d, 10 mg? kg -1), AL-IMTA (120.3 mg? kg -1) were intraperitoneal injected since Day 1(every three days, 3 times in total).After the trial, calculated inhibition rate against tumor , determined peripheral hemogram and recorded body weight before /after administration , body weight ratio(FBW/IBW) and death.Results: Compared with the model group, steamed Panax notoginseng(1170 mg? kg-1) showed significantl inhibition rate(41%, P<0.01);raw Panax notoginseng(2340 mg? kg-1) also showed significantl inhibition rate (P<0.05 ) , but with no clinical significance , and had several adverse reactions .Steamed and raw Panax notoginseng improved the inhibition rate of paclitaxel and ALIMTA;Steamed Panax notoginseng seemed to reduce the toxicity of paclitaxel , whereas raw Pa-nax notoginseng increased the toxicity of chemotherapy drugs .Conclusion: Steamed Panax notoginseng significantly inhibit the growth of Lewis lung cancer; and when use in combination with chemotherapy drugs , could improve the inhibition rate , mean-while , reduce the toxicity of chemotherapy drugs .There is no clinical significance of antitumor effect of raw Panax notoginseng ;al-though raw Panax notoginseng could also improve the inhibition rate of chemotherapy drugs , but exhibit obvious adverse reactions .
