CAJ | 학술논문

目的：观察醒脑静注射液对脓毒症大鼠心肌线粒体氧化应激的影响。方法：以腹腔注射脂多糖（LPS）建立脓毒症模型，胃管注入醒脑静，将70只SD 大鼠随机分为7组，每组10只：脓毒症6 h 组（LPS 6 h组）、LPS 24 h组、LPS 48 h 组、醒脑静治疗6 h 组（XNJ 6 h 组）、XNJ 24 h 组、XNJ 48 h 组、对照组。观察各组不同时点心肌线粒体电镜下形态改变、半定量评分和超氧化物歧化酶（SOD）、丙二醛（MDA）、一氧化氮（NO）、一氮化氮合酶（iNOS）水平的动态变化。结果：醒脑静注射液可改善脓毒症大鼠心肌线粒体电镜下的形态改变，降低心肌线粒体电镜下的半定量评分，并可显著降低脓毒症大鼠心肌线粒体 MDA、NO、iNOS 水平和提高脓毒症大鼠心肌线粒体SOD水平。结论：脑静注射液可显著降低脓毒症大鼠心肌线粒体氧化应激的水平和提高清除氧自由基的能力，从而起到保护脓毒症大鼠心肌线粒体的作用。
목적：관찰성뇌정주사액대농독증대서심기선립체양화응격적영향。방법：이복강주사지다당（LPS）건립농독증모형，위관주입성뇌정，장70지SD 대서수궤분위7조，매조10지：농독증6 h 조（LPS 6 h조）、LPS 24 h조、LPS 48 h 조、성뇌정치료6 h 조（XNJ 6 h 조）、XNJ 24 h 조、XNJ 48 h 조、대조조。관찰각조불동시점심기선립체전경하형태개변、반정량평분화초양화물기화매（SOD）、병이철（MDA）、일양화담（NO）、일담화담합매（iNOS）수평적동태변화。결과：성뇌정주사액가개선농독증대서심기선립체전경하적형태개변，강저심기선립체전경하적반정량평분，병가현저강저농독증대서심기선립체 MDA、NO、iNOS 수평화제고농독증대서심기선립체SOD수평。결론：뇌정주사액가현저강저농독증대서심기선립체양화응격적수평화제고청제양자유기적능력，종이기도보호농독증대서심기선립체적작용。
Objective To observe the effect of Xingnaojing injection in myocardial mitochondrial oxidative stress injury in sepsis mice. Methods The septic model were set up by receiving endotoxin through interaperitoneal injection. After Xingnaojing injection by gastric tube , seventy mice were randomly divided into 7 groups in ten of each group including group LPS-6 h , group LPS-24 h , group LPS-48 h; group XNJ-6 h , group XNJ-24 h, group XNJ-48 h and control group. The myocardial mitochondrial changes , the semi-quantitative scores and the level of SOD、MDA、NO、iNOS in sepsis mice were observed. Results Xingnaojing injection could improve the myocardial mitochondrial changes , reduce the semi-quantitative scores , significantly reduce the level of MDA、NO、iNOS and elevate the level of SOD. Conclusion Xingnaojing injection could significantly reduce the myocardial mitochondrial oxidative stress level and improve the ability of clearing oxygen radicals , thereby protect the myocardial mitochondrion in sepsis mice.