CAJ | 학술논문

目的：对比生、熟三七粉对环磷酰胺所致血虚小鼠的治疗作用，并研究其机理。方法：108只KM小鼠按体重随机分为空白组，模型组，阳性组，生、熟三七低、中、高剂量组，每组12只。其中生、熟三七粉按低、中、高剂量（585 mg? kg－1，1170 mg? kg－1，2340 mg? kg－1）、阳性组以8 mL? kg－1剂量灌胃复方阿胶浆，连续12 d，自给药起前3 d以70 mg? kg－1剂量连续腹腔注射环磷酰胺生理盐水溶液制作血虚模型，试验结束后以外周WBC、RBC、Hb、PLT及脏器指数（脾脏、胸腺）为指标对生、熟三七补血作用进行比较；并以流式细胞技术测定骨髓DNA含量及骨髓细胞CD34＋的表达，结合CFU－GM、BFU－E、CFU－E、CFU－Meg的培养研究熟三七的补血机制。结果：与空白组相比，模型组小鼠外周WBC、RBC、HB及PLT、脏器指数均有极显著下降（ P＜0．01），骨髓DNA含量及CD34＋均显著下降（ P＜0．01）；与模型组相比，熟三七高、中、低剂量组均能显著升高外周WBC、RBC、Hb、PLT（ P＜0．05），并均能显著提高脏器指数（胸腺、脾脏）（ P＜0．05），同等剂量生三七则无明显效果；与模型组相比，熟三七组骨髓DNA及细胞CD34＋表达率均有显著升高（P＜0．05），CFU－GM、BFU－E、CFU－E、CFU－Meg集落数均有显著增加（ P＜0．05）。结论：熟三七可通过促进骨髓细胞增殖而达到补血的功效。
목적：대비생、숙삼칠분대배린선알소치혈허소서적치료작용，병연구기궤리。방법：108지KM소서안체중수궤분위공백조，모형조，양성조，생、숙삼칠저、중、고제량조，매조12지。기중생、숙삼칠분안저、중、고제량（585 mg? kg－1，1170 mg? kg－1，2340 mg? kg－1）、양성조이8 mL? kg－1제량관위복방아효장，련속12 d，자급약기전3 d이70 mg? kg－1제량련속복강주사배린선알생리염수용액제작혈허모형，시험결속후이외주WBC、RBC、Hb、PLT급장기지수（비장、흉선）위지표대생、숙삼칠보혈작용진행비교；병이류식세포기술측정골수DNA함량급골수세포CD34＋적표체，결합CFU－GM、BFU－E、CFU－E、CFU－Meg적배양연구숙삼칠적보혈궤제。결과：여공백조상비，모형조소서외주WBC、RBC、HB급PLT、장기지수균유겁현저하강（ P＜0．01），골수DNA함량급CD34＋균현저하강（ P＜0．01）；여모형조상비，숙삼칠고、중、저제량조균능현저승고외주WBC、RBC、Hb、PLT（ P＜0．05），병균능현저제고장기지수（흉선、비장）（ P＜0．05），동등제량생삼칠칙무명현효과；여모형조상비，숙삼칠조골수DNA급세포CD34＋표체솔균유현저승고（P＜0．05），CFU－GM、BFU－E、CFU－E、CFU－Meg집락수균유현저증가（ P＜0．05）。결론：숙삼칠가통과촉진골수세포증식이체도보혈적공효。
Objective:To compare the different effect between raw and steamed panax notoginseng powder on deficiency of blood caused by cyclophosphamide , and its mechanism.Methods:A total of 108 KM mice were randomly divided by weight into:nor-mal group, model group, positive group, low, medium and high dose group of raw and steamed panax notoginseng powder ,12 mice in each group .The raw and steamed groups were given three different dose of panax notoginseng powder ( 585 ,1170 ,2340 mg? kg -1 ) , the positive group was given with 8 mL? kg-1 Compound E-Jiao slurry ,lavage for 12 days.The deficiency of blood model were made in the first three days by intraperitoneal injection of cyclophosphamide with the concentration of 70 mg? kg-1 in mice.After the experiment , we evaluated the effectiveness of drugs by WBC , RBC, Hb, PLT and organ index ( spleen and thy-mus) .Then flow cytometry was used to measure DNA content of bone marrow cells and CD 34 +expression, conbined with the cul-ture of CFU-GM, BFU-E, CFU-E, CFU-Meg to research the blood enriching function of steamed panax notoginseng powder .Re-sults:Compared with the normal group , the WBC, RBC, Hb , PLT and the organ index ( spleen and thymus ) of model group were significantly decreased ( P<0.01 ) , DNA content of bone marrow cells and CD 34 +expression were significantly decreased (P<0.01);Compared with model group , the high, medium and low dose group of steamed panax notoginseng powder were all significantly increased by WBC , RBC, Hb, PLT ( P<0.05 ) , and can significantly increase the organs index thymus and spleen ( P<0.05 ) , the same dose of raw panax notoginseng had no obvious effect;Compared with model group , the DNA content of bone marrow cells and CD34 +expression of steamed panax notoginseng were significantly higher (P<0.05), CFU-GM, BFU-E, CFU-E, CFU-Meg colony forming units were significantly increased too (P<0.05).Conclusion:Steamed panax notoginseng can exert the blood enriching function by promoting the proliferation of bone marrow cells .