CAJ | 학술논문

目的：探讨利用高效液相色谱法对蒸制三七粉中皂苷类成分的测定。方法：采用高效液相色谱法，以十八烷基硅烷键合硅胶为填充剂，乙腈－水为流动相进行梯度洗脱，建立同时分析16个三七皂苷对照品Rg1、Re、Rb1、Rc、Rb3、Rd、Rg3、Rh1、Rg6、F4、Rh4、Rk3、Rk1、Rg5等的方法，并对蒸制三七粉样品进行成分测定。结果：通过对蒸制三七粉样品HPLC图与混合样品的HPLC图各峰保留时间进行对比，发现样品中含有未炮制三七不含的Rg3、Rh1、Rg6、F4、Rh4、Rk3、Rk1、Rg5皂苷类成分。结论：利用高效液相色谱法对蒸制三七粉成分进行分析确证其中含有提升免疫的成分，符合三七“生消熟补”的理论，对下一步该饮片指纹图谱研究奠定了基础。
목적：탐토이용고효액상색보법대증제삼칠분중조감류성분적측정。방법：채용고효액상색보법，이십팔완기규완건합규효위전충제，을정－수위류동상진행제도세탈，건립동시분석16개삼칠조감대조품Rg1、Re、Rb1、Rc、Rb3、Rd、Rg3、Rh1、Rg6、F4、Rh4、Rk3、Rk1、Rg5등적방법，병대증제삼칠분양품진행성분측정。결과：통과대증제삼칠분양품HPLC도여혼합양품적HPLC도각봉보류시간진행대비，발현양품중함유미포제삼칠불함적Rg3、Rh1、Rg6、F4、Rh4、Rk3、Rk1、Rg5조감류성분。결론：이용고효액상색보법대증제삼칠분성분진행분석학증기중함유제승면역적성분，부합삼칠“생소숙보”적이론，대하일보해음편지문도보연구전정료기출。
Objective:Using high-pressure liquid chromatography ( HPLC) method to determine the saponin constituents of steamed Panax notoginseng .Methods:By HPLC, with 18 alkyl silane bonded silica as filler , and acetonitrile-water as mobile phase for gradient elution , we established a method that could simultaneously analyze 16 notoginsenoside controls ( Rg1 , Re, Rb1 , Rc, Rb3 , Rd, Rg3 , Rh1 , Rg6 , F4 , Rh4 , Rk3 , Rk1 , Rg5 ) , and measured the constituents of steamed Panax notoginseng .Results: By comparing each peak 's retention time of the steamed Panax notoginseng HPLC map with mixed samples HPLC map , we found sev-eral saponin constituents ( Rg3 , Rh1 , Rg6 , F4 , Rh4 , Rk3 , Rk1 , Rg5 ) which are not produced by unsteamed Panax notoginseng . Conclusion:By HPLC analysis , we confirmed that steamed Panax notoginseng contain several immune-enhancing constituents , which is in accordance with the traditional Chinese medicine 's theory about Panax notoginseng .This research lay the foundation of future fingerprint research of Panax notoginseng .