CAJ | 학술논문

目的：初步探讨低密度脂蛋白受体相关蛋白-1(LRP1)依赖的信号通路对轴突生长及神经损伤修复的作用。方法在培养胎鼠皮层神经元过程中加入LRP1激动剂RBD，观察其与对照组相比能否促进神经突触的生长及其信号机制，此外通过制作小鼠脊髓损伤模型并予以鞘内注射RBD，观察其对神经损伤修复的影响。结果体外培养皮层神经元中加入RBD后引起神经营养因子受体TrkC，Akt信号转导通路和细胞外调节蛋白激酶(ERK)的活化，其与对照组相比定量分析表达均明显升高(P<0.05)，促进神经突的生长。鞘内注射RBD后小鼠脊髓损伤后的运动功能恢复评分也明显提高(P<0.05)。结论激活LRP1依赖的信号通路可明显促进轴突生长及小鼠脊髓损伤后的功能修复。
목적：초보탐토저밀도지단백수체상관단백-1(LRP1)의뢰적신호통로대축돌생장급신경손상수복적작용。방법재배양태서피층신경원과정중가입LRP1격동제RBD，관찰기여대조조상비능부촉진신경돌촉적생장급기신호궤제，차외통과제작소서척수손상모형병여이초내주사RBD，관찰기대신경손상수복적영향。결과체외배양피층신경원중가입RBD후인기신경영양인자수체TrkC，Akt신호전도통로화세포외조절단백격매(ERK)적활화，기여대조조상비정량분석표체균명현승고(P<0.05)，촉진신경돌적생장。초내주사RBD후소서척수손상후적운동공능회복평분야명현제고(P<0.05)。결론격활LRP1의뢰적신호통로가명현촉진축돌생장급소서척수손상후적공능수복。
Objective To investigate the effects of the low density lipoprotein receptors-1 (LRP1) dependent signal pathway in axonal growth and nerve regeneration after injury. Methods Adding RBD, LRP1 agonist, when culture the cortical neurons of fetal rats, observing whether it can promote the growth of synapses and what the signal mechanism it is compared with the control group. In addition, observing the effects of RBD on the repair of nerve injury through building the spinal cord injury model of rats up and intrathecal inject the RBD. Results The activation of neurotrophic factor receptor TrkC, Akt signal transduction pathway and extracellular regulated protein kinase (ERK) which were induced after add RBD in cortical neuron in vitro. The results of quantitative analysis of expression between the intervention group and the control group were significantly increased (P<0.05), the growth of neurite were promoted. The recovery of motor function after spinal cord injury scores were significantly improved after the intrathecal injection of RBD in mice. Conclusion The activation of signal pathway dependented by LRP1 can obviously promote the growth of axon and repair functions of rats after the spinal injury.