CAJ | 학술논문

为了研究桑叶多糖不同组分在体外的抗凝血活性,采用水提醇沉法提取桑叶粗多糖,聚酰胺进行脱色和脱蛋白。用DEAE sepharose CL-6B 和 sepharose CL-6B 对桑叶多糖进行纯化,得到5种水溶性多糖组分(MPS-1、MPS-2a、MPS-2b、MPS-2c和 MPS-3)。单糖检测结果表明：桑叶多糖主要是由半乳糖、葡萄糖、鼠李糖、阿拉伯糖、木糖和甘露糖等组成。体外抗凝血活性检测显示5种桑叶多糖组分均能延长活化部分凝血活酶时间(activated partial thromboplastin time,APTT)和凝血酶时间(thrombin time,TT)、而对凝血酶原时间(prothrombin time,PT)影响不显著,说明它们是通过影响内源性途径或共同途径而发挥抗凝血作用；与对照相比,MPS-2b在试验浓度范围内对 APTT 和 TT 的效果达到极显著,表明 MPS-2b 有潜力开发成抗凝血活性物质。
위료연구상협다당불동조분재체외적항응혈활성,채용수제순침법제취상협조다당,취선알진행탈색화탈단백。용DEAE sepharose CL-6B 화 sepharose CL-6B 대상협다당진행순화,득도5충수용성다당조분(MPS-1、MPS-2a、MPS-2b、MPS-2c화 MPS-3)。단당검측결과표명：상협다당주요시유반유당、포도당、서리당、아랍백당、목당화감로당등조성。체외항응혈활성검측현시5충상협다당조분균능연장활화부분응혈활매시간(activated partial thromboplastin time,APTT)화응혈매시간(thrombin time,TT)、이대응혈매원시간(prothrombin time,PT)영향불현저,설명타문시통과영향내원성도경혹공동도경이발휘항응혈작용；여대조상비,MPS-2b재시험농도범위내대 APTT 화 TT 적효과체도겁현저,표명 MPS-2b 유잠력개발성항응혈활성물질。
To study different components of mulberry polysaccharides and their anticoagulant activity,the crude mulberry polysaccharides were extracted by water and precipitated by alcohol.The crude polysac-charides were decolorized and deproteinized by polyamide.Five water-soluble polysaccharides (MPS-1 , MPS-2a,MPS-2b,MPS-2c and MPS-3)were purified by DEAE sepharose CL-6B chromatograph column and sepharose CL-6B chromatograph column.The monosaccharide compostion of MPS was composed mainly of galactose,glucose,rhamnose,arabinose,xylose and mannose and so on.APTT,PT and TT in vitro tests showed that they prolonged APTT and TT,but had no significant effect on PT.The result confirmed these in the regulation of coagulation initiated via the intrinsic pathway or common pathway;MPS-2b significantly prolonged APTT and TT compared to saline in experiment concentrations.These results suggest that the mulberry polysaccharide MPS-2b can be explored as a kind of natural potential anticoagulant.