CAJ | 학술논문

酶的固定化是提高酶的稳定及降低使用成本的重要途径．通过制备聚乙烯醇(PVA)-海藻酸钠(SA)复合载体,对共固定化葡萄糖氧化酶(GOD)和过氧化氢酶(CAT)的条件进行了研究,优化了固定化酶制备工艺,研究了固定化酶性质．得出制备固定化酶最佳条件为：载体比例 PVA∶SA＝9．0∶1．5,加酶量10 mg/mL,酶活之比CAT∶GOD＝10∶1．固定化酶的最适反应温度为45℃,比游离酶提高了5℃,最适反应pH 没有发生变化,连续使用6次酶活保留60％．研究结果有一定的应用潜力．
매적고정화시제고매적은정급강저사용성본적중요도경．통과제비취을희순(PVA)-해조산납(SA)복합재체,대공고정화포도당양화매(GOD)화과양화경매(CAT)적조건진행료연구,우화료고정화매제비공예,연구료고정화매성질．득출제비고정화매최가조건위：재체비례 PVA∶SA＝9．0∶1．5,가매량10 mg/mL,매활지비CAT∶GOD＝10∶1．고정화매적최괄반응온도위45℃,비유리매제고료5℃,최괄반응pH 몰유발생변화,련속사용6차매활보류60％．연구결과유일정적응용잠력．
The immobilization is an effective way to improve the stability and reduce the cost of the en-zymes.The co-immobilization of glucose oxidase and catalase was studied through the preparation of composite support of the polyvinyl alcohol-sodium alginate with optimized the preparation of immobi-lized enzyme,the characters of immobilized enzyme were investigated.The preparation conditions of im-mobilized enzyme were as follows:the ration of PVA∶SA=9 .0∶1 .5 ,the amount of enzyme was 10 mg/mL,and the ratio enzyme activity of CAT∶GOD=10∶1.The optimum reaction temperature was 45 ℃,5 ℃ higher than the free enzyme,the optimal reaction pH remained unchanged,and the activity re-tained 60% after being used six times.The research results are promising in applications.