上海预防医学
上海預防醫學
상해예방의학
SHANGHAI JOURNAL OF PREVENTIVE MEDICINE
2015年
5期
283-287
,共5页
温忆敏%林元杰%朱怡平%金玉娥
溫憶敏%林元傑%硃怡平%金玉娥
온억민%림원걸%주이평%금옥아
内标法%基质效应%高效液相色谱-串联质谱法%甜蜜素
內標法%基質效應%高效液相色譜-串聯質譜法%甜蜜素
내표법%기질효응%고효액상색보-천련질보법%첨밀소
Internal standard method%Matrix effect%HPLC-tandem mass spectrometry%Cyclamate
[目的]探讨液相色谱-质谱联用法测定酒中甜蜜素的内标适用性。[方法]酒样直接稀释后,采用液相色谱-质谱联用法测定,并分别用外标法和内标法对结果进行定量。[结果]2种定量方法的结果平均相差11.2%,最大相对相差为16.2%,而外标定量法的结果与国标方法GB/T 5009.97—2003测定的结果一致。[结论]结果差异源于液质联用的基质效应及其内标化合物选择的不适。
[目的]探討液相色譜-質譜聯用法測定酒中甜蜜素的內標適用性。[方法]酒樣直接稀釋後,採用液相色譜-質譜聯用法測定,併分彆用外標法和內標法對結果進行定量。[結果]2種定量方法的結果平均相差11.2%,最大相對相差為16.2%,而外標定量法的結果與國標方法GB/T 5009.97—2003測定的結果一緻。[結論]結果差異源于液質聯用的基質效應及其內標化閤物選擇的不適。
[목적]탐토액상색보-질보련용법측정주중첨밀소적내표괄용성。[방법]주양직접희석후,채용액상색보-질보련용법측정,병분별용외표법화내표법대결과진행정량。[결과]2충정량방법적결과평균상차11.2%,최대상대상차위16.2%,이외표정량법적결과여국표방법GB/T 5009.97—2003측정적결과일치。[결론]결과차이원우액질련용적기질효응급기내표화합물선택적불괄。
Objective] To discuss the applicability of internal standard in determination of cycla-mate in wine by HPLC-tandem mass spectrometry . [ Methods] After dilution wine sample was deter-mined by HPLC-tandem mass spectrometry and quantified by external and internal standard methods respec-tively. [Results] The results by the 2 quantitation methods differed 11.2%in average, and the maxi-mum relative deviation was 16.2%.And the result by external standard quantitation method proved to be consistent with that by the international standard GB/T 5009.97-2003. [ Conclusion] The difference in results is thought to originate from matrix effect in HPLC-tandem mass spectrometry and the improper in-ternal standard compound applied .