中国康复理论与实践
中國康複理論與實踐
중국강복이론여실천
CHINESE JOURNAL OF REHABILITATION THEORY & PRACTICE
2015年
5期
519-523
,共5页
王玉昌%庞伟%冯欢欢%刘师振%张慧
王玉昌%龐偉%馮歡歡%劉師振%張慧
왕옥창%방위%풍환환%류사진%장혜
痉挛型瘫痪%甲基化%超微结构%Ⅰ型肌球蛋白重链
痙攣型癱瘓%甲基化%超微結構%Ⅰ型肌毬蛋白重鏈
경련형탄탄%갑기화%초미결구%Ⅰ형기구단백중련
spastic paralysis%methylation%ultrastructure%myosin heavy chain-I
目的：探讨骨骼肌在痉挛状态下对DNA甲基化水平的影响及与肌纤维构型的相关性。方法健康5日龄Wistar新生仔鼠100只，随机分为模型组和对照组。前者制备痉挛型瘫痪大鼠模型成功后饲养30 d。对两组大鼠腓肠肌进行肌肉活检。分别进行骨骼肌DNA甲基化水平测定、骨骼肌Ⅰ型肌球蛋白重链mRNA半定量RT-PCR检测，透射电镜观察。结果模型组骨骼肌总体DNA甲基化水平(4.95±0.83)×10%，显著低于对照组的(6.59±0.75)×10%(P<0.001)；模型组骨骼肌Ⅰ型肌球蛋白重链mRNA表达量(1.23±0.31)，显著高于对照组的(0.44±0.29)(P<0.001)。电镜观察，模型组骨骼肌Z线排列不规整，两旁骨骼肌线粒体增多，线粒体肿胀，线粒体嵴部分断裂；粗细肌丝数量关系失衡，肌原纤维包膜融合，有的包膜间隙增宽。结论痉挛型瘫痪大鼠骨骼肌DNA低甲基化，Ⅰ型肌球蛋白重链mRNA高表达；电镜检测以Ⅰ型纤维表现为主。没有充足证据表明骨骼肌DNA甲基化水平与肌纤维构型改变相关。
目的：探討骨骼肌在痙攣狀態下對DNA甲基化水平的影響及與肌纖維構型的相關性。方法健康5日齡Wistar新生仔鼠100隻，隨機分為模型組和對照組。前者製備痙攣型癱瘓大鼠模型成功後飼養30 d。對兩組大鼠腓腸肌進行肌肉活檢。分彆進行骨骼肌DNA甲基化水平測定、骨骼肌Ⅰ型肌毬蛋白重鏈mRNA半定量RT-PCR檢測，透射電鏡觀察。結果模型組骨骼肌總體DNA甲基化水平(4.95±0.83)×10%，顯著低于對照組的(6.59±0.75)×10%(P<0.001)；模型組骨骼肌Ⅰ型肌毬蛋白重鏈mRNA錶達量(1.23±0.31)，顯著高于對照組的(0.44±0.29)(P<0.001)。電鏡觀察，模型組骨骼肌Z線排列不規整，兩徬骨骼肌線粒體增多，線粒體腫脹，線粒體嵴部分斷裂；粗細肌絲數量關繫失衡，肌原纖維包膜融閤，有的包膜間隙增寬。結論痙攣型癱瘓大鼠骨骼肌DNA低甲基化，Ⅰ型肌毬蛋白重鏈mRNA高錶達；電鏡檢測以Ⅰ型纖維錶現為主。沒有充足證據錶明骨骼肌DNA甲基化水平與肌纖維構型改變相關。
목적：탐토골격기재경련상태하대DNA갑기화수평적영향급여기섬유구형적상관성。방법건강5일령Wistar신생자서100지，수궤분위모형조화대조조。전자제비경련형탄탄대서모형성공후사양30 d。대량조대서비장기진행기육활검。분별진행골격기DNA갑기화수평측정、골격기Ⅰ형기구단백중련mRNA반정량RT-PCR검측，투사전경관찰。결과모형조골격기총체DNA갑기화수평(4.95±0.83)×10%，현저저우대조조적(6.59±0.75)×10%(P<0.001)；모형조골격기Ⅰ형기구단백중련mRNA표체량(1.23±0.31)，현저고우대조조적(0.44±0.29)(P<0.001)。전경관찰，모형조골격기Z선배렬불규정，량방골격기선립체증다，선립체종창，선립체척부분단렬；조세기사수량관계실형，기원섬유포막융합，유적포막간극증관。결론경련형탄탄대서골격기DNA저갑기화，Ⅰ형기구단백중련mRNA고표체；전경검측이Ⅰ형섬유표현위주。몰유충족증거표명골격기DNA갑기화수평여기섬유구형개변상관。
Objective To investigate the DNA methylation of skeletal muscle in spastic paralysis rats and correlation with the muscle fi-ber configuration. Methods 100 5-day old Wistar rats were randomly divided into model group and control group. The former was estab-lished the spastic paralysis modle and reared for 30 days. Then, tissues from the gastrocnemius of all the rats were observed with triplicate DNA methylation, myosin heavy chain-I (MHC-I) mRNA with RT-PCR and transmission electron microscopy. Results The DNA methyla-tion was (4.95 ± 0.83) × 10%in the model group, significantly less than (6.59 ± 0.75) × 10%in the control group (P<0.001);while the MHC-I mRNA was (1.23±0.31), significantly more than (0.44±0.29) in the control group (P<0.001). The Z-line was disordered, and the mitochon-dria near the Z-line increased, with edema and partially broken in cristae. The balance between the thick and thin filaments was broken, and myofibrils envelope fused. Conclusion Hypomethylation and hyperexpression of MHC-I mRNA have been found in skeletal muscle of spas-tic paralysis rats, which may result in type I fibers increase. However, there was no sufficient evidence to support the correlation between the DNA methylation and the secondary pathological changes.
