CAJ | 학술논문

目的：探索时钟基因启动子甲基化频率增龄性改变及其在衰老中的作用。方法4月龄(青年组，n=9)、20月龄(老年组，n=10) C57BL小鼠，采用甲基化特异性聚合酶链反应方法检测小鼠胃、脾、血管、肾、纹状体中时钟基因Per1/2、Bmal1/2、Cry1/2、Clock、Npas2启动子甲基化水平。结果老年组脾组织Cry1、Bmal2和NPas2启动子区甲基化频率高于青年组(P<0.05)，胃组织Per1基因启动子甲基化频率低于青年组(P<0.05)，血管组织Bmal1启动子区甲基化频率高于青年组(P<0.05)。结论多种时钟基因启动子甲基化可能参与衰老进程，并具有组织特异性。
목적：탐색시종기인계동자갑기화빈솔증령성개변급기재쇠로중적작용。방법4월령(청년조，n=9)、20월령(노년조，n=10) C57BL소서，채용갑기화특이성취합매련반응방법검측소서위、비、혈관、신、문상체중시종기인Per1/2、Bmal1/2、Cry1/2、Clock、Npas2계동자갑기화수평。결과노년조비조직Cry1、Bmal2화NPas2계동자구갑기화빈솔고우청년조(P<0.05)，위조직Per1기인계동자갑기화빈솔저우청년조(P<0.05)，혈관조직Bmal1계동자구갑기화빈솔고우청년조(P<0.05)。결론다충시종기인계동자갑기화가능삼여쇠로진정，병구유조직특이성。
Objective To investigate the role of the clock gene promoter methylation in aging. Methods C57BL mice of 4-(young, n=9) and 20-(old, n=10) month-old were determined the promoter methylation level of clock genes (Per1/2, Bmal1/2, Cry1/2, Clock, Npas2) in the stomach, spleen, vascular, kidney and striatum with methylation-specific polymerase chain reaction (MSP). Results The incidence of promoter methylation of Cry1, Bmal2 and Npas2 in spleen increased in old mice (P<0.05), while the promoter methylation of Per1 in stom-ach decreased (P<0.05), and the promoter methylation of Bmal1 in vascular increased (P<0.05). Conclusion Promoter methylation of some clock genes is involved in process of aging in a tissue-specific way.