棉花科学
棉花科學
면화과학
JIANGXI COTTONS
2015年
3期
7-12
,共6页
刘超%张俊%姚正培%倪志勇%陈全家
劉超%張俊%姚正培%倪誌勇%陳全傢
류초%장준%요정배%예지용%진전가
海岛棉%黄烷酮3-羟化酶基因%序列分析%基因表达
海島棉%黃烷酮3-羥化酶基因%序列分析%基因錶達
해도면%황완동3-간화매기인%서렬분석%기인표체
Gossypium barbadense%Flavanone 3-hydroxylase%Sequential analysis%Gene expression
根据棉纤维发育相关基因转录组学、表达谱分析结果，从海岛棉（新海21）中筛选到一个与棉纤维发育相关的黄烷酮3－羟化酶基因（GbF3H）。生物信息学分析表明，该基因编码区长1107bp，编码368个氨基酸，多序列比对发现 GbF3H序列保守性较高，具有典型的2－酮戊二酸双加氧酶结构域。实时定量PCR分析表明，GbF3H基因在纤维发育的不同时期中均有表达，在开花后5 d的胚珠中表达量最高，推测GbF3H基因可能对棉纤维发育具有重要作用。
根據棉纖維髮育相關基因轉錄組學、錶達譜分析結果，從海島棉（新海21）中篩選到一箇與棉纖維髮育相關的黃烷酮3－羥化酶基因（GbF3H）。生物信息學分析錶明，該基因編碼區長1107bp，編碼368箇氨基痠，多序列比對髮現 GbF3H序列保守性較高，具有典型的2－酮戊二痠雙加氧酶結構域。實時定量PCR分析錶明，GbF3H基因在纖維髮育的不同時期中均有錶達，在開花後5 d的胚珠中錶達量最高，推測GbF3H基因可能對棉纖維髮育具有重要作用。
근거면섬유발육상관기인전록조학、표체보분석결과，종해도면（신해21）중사선도일개여면섬유발육상관적황완동3－간화매기인（GbF3H）。생물신식학분석표명，해기인편마구장1107bp，편마368개안기산，다서렬비대발현 GbF3H서렬보수성교고，구유전형적2－동무이산쌍가양매결구역。실시정량PCR분석표명，GbF3H기인재섬유발육적불동시기중균유표체，재개화후5 d적배주중표체량최고，추측GbF3H기인가능대면섬유발육구유중요작용。
According to analysis of expression profiling and transcriptome during cotton fiber development,Screening to a cotton fiber development related flavanone 3 -hydroxylase gene (GbF3H)in gossypium barbadense (Xinhai 21 ),Bioinfor-matics analysis indicated that the length of open reading frame (ORF)in GbF3H gene was 1 1 07bp,it could encode a protein which comprising 368 amino acids . Multiple sequence alignment revealed that the GbF3H gene had a high conservative in se-quence . In addition,it had the typical characteristics of 2-ODD conservative structure domain . Real-time PCR analysis showed that GbF3H genes could express in the fiber development at different periods . It had the highest expression in ovule at the 5th day after flowering. These results suggested that GbF3H gene should play an important role in the fiber development.
