中华神经科杂志
中華神經科雜誌
중화신경과잡지
Chinese Journal of Neurology
2015年
5期
368-372
,共5页
胡凯凯%池丽芬%易兴阳%邵蓓
鬍凱凱%池麗芬%易興暘%邵蓓
호개개%지려분%역흥양%소배
脑缺血%卒中%环氧水解酶类%多态性,单核苷酸%突变
腦缺血%卒中%環氧水解酶類%多態性,單覈苷痠%突變
뇌결혈%졸중%배양수해매류%다태성,단핵감산%돌변
Brain ischemia%Stroke%Epoxide hydrolases%Polymorphism,single nucleotide%Mutation
目的 探索浙江南部地区人群白三烯A4水解酶(LTA4H)基因中rs2660845、rs2540493单核苷酸多态性与缺血性卒中的关系.方法 选取2010年9月至2013年6月在温州医科大学附属第三医院神经内科住院的300例脑梗死患者,另选该院同期300名健康体检者作对照.采用PCR和激光解析/电离飞行时间质谱法,分析LTA4H基因中rs2660845、rs2540493位点的单核苷酸多态性.采用完全随机化方法从中随机选取67例脑梗死患者及30名对照者,采用ELISA法检测血清白三烯B4水平.结果 LTA4H基因的rs2660845、rs2540493的突变与卒中风险或Org 10 172急性卒中治疗试验(TOAST)分型均无明显相关性(P＞0.05).分析血清白三烯B4水平显示,血清白三烯B4浓度在脑梗死组(n=67,0.991±0.305)与对照组(n=30,1.035±0.498)之间差异无统计学意义(P=0.692),也未发现其在rs2660845位点的各基因型之间差异有统计学意义(AG基因型0.938±0.269,AA基因型1.038±0.268,GG基因型1.043±0.383;P=0.401).结论 LTA4H基因中rs2660845、rs2540493位点的单核苷酸多态性与浙江南部地区人群的缺血性卒中风险无相关性.
目的 探索浙江南部地區人群白三烯A4水解酶(LTA4H)基因中rs2660845、rs2540493單覈苷痠多態性與缺血性卒中的關繫.方法 選取2010年9月至2013年6月在溫州醫科大學附屬第三醫院神經內科住院的300例腦梗死患者,另選該院同期300名健康體檢者作對照.採用PCR和激光解析/電離飛行時間質譜法,分析LTA4H基因中rs2660845、rs2540493位點的單覈苷痠多態性.採用完全隨機化方法從中隨機選取67例腦梗死患者及30名對照者,採用ELISA法檢測血清白三烯B4水平.結果 LTA4H基因的rs2660845、rs2540493的突變與卒中風險或Org 10 172急性卒中治療試驗(TOAST)分型均無明顯相關性(P＞0.05).分析血清白三烯B4水平顯示,血清白三烯B4濃度在腦梗死組(n=67,0.991±0.305)與對照組(n=30,1.035±0.498)之間差異無統計學意義(P=0.692),也未髮現其在rs2660845位點的各基因型之間差異有統計學意義(AG基因型0.938±0.269,AA基因型1.038±0.268,GG基因型1.043±0.383;P=0.401).結論 LTA4H基因中rs2660845、rs2540493位點的單覈苷痠多態性與浙江南部地區人群的缺血性卒中風險無相關性.
목적 탐색절강남부지구인군백삼희A4수해매(LTA4H)기인중rs2660845、rs2540493단핵감산다태성여결혈성졸중적관계.방법 선취2010년9월지2013년6월재온주의과대학부속제삼의원신경내과주원적300례뇌경사환자,령선해원동기300명건강체검자작대조.채용PCR화격광해석/전리비행시간질보법,분석LTA4H기인중rs2660845、rs2540493위점적단핵감산다태성.채용완전수궤화방법종중수궤선취67례뇌경사환자급30명대조자,채용ELISA법검측혈청백삼희B4수평.결과 LTA4H기인적rs2660845、rs2540493적돌변여졸중풍험혹Org 10 172급성졸중치료시험(TOAST)분형균무명현상관성(P＞0.05).분석혈청백삼희B4수평현시,혈청백삼희B4농도재뇌경사조(n=67,0.991±0.305)여대조조(n=30,1.035±0.498)지간차이무통계학의의(P=0.692),야미발현기재rs2660845위점적각기인형지간차이유통계학의의(AG기인형0.938±0.269,AA기인형1.038±0.268,GG기인형1.043±0.383;P=0.401).결론 LTA4H기인중rs2660845、rs2540493위점적단핵감산다태성여절강남부지구인군적결혈성졸중풍험무상관성.
Objective To investigate the correlation between two single nucleotide polymorphisms of the leukotriene A4 hydrolase (LTA4H) gene (rs2660845 and rs2540493) and risk of ischemic stroke in population of southern Zhejiang Province.Methods A total of 300 ischemic stroke patients and 300 healthy controls,recruited from the Department of Neurology,Third Affiliated Hospital of Wenzhou Medical University between September 2010 and June 2013,were enrolled in this study.Two single nucleotide polymorphisms of the LTA4H gene (rs2660845 and rs2540493) were analyzed by polymerase chain reaction and matrix-assisted laser desorption/ionization time of flight,respectively.Sixty-seven patients and thirty controls were randomly selected (complete randomization) and detected the serum leukotriene B4 (LTB4)concentration by ELISA method.Results There was no evidence of association between the two variants of LTA4H gene and the risk of ischemic stroke or its TOAST (Trial of Org 10 172 in acute stroke treatment)subtypes (P ＞ 0.05).Analysis of LTB4 levels revealed that there was no statistically significant difference in serum LTB4 concentration between patients (n =67) and controls (n =30; 0.991 ± 0.305 vs 1.035 ± 0.498 ; P =0.692),and no statistically significant difference in LTB4 concentration was found among the three genotypes of rs2660845 as well (AG genotype vs AA genotype vs GG genotype:0.938 ± 0.269 vs 1.038 ± 0.268 vs 1.043 ± 0.383 ; P =0.401).Conclusion The present study suggests that there is no association between the two polymorphisms in the LTA4H gene and risk of ischemic stroke in population of southern Zhejiang Province.
