CAJ | 학술논문

[目的]总结手足口病（ HFMD）患儿外周血T淋巴细胞、B淋巴细胞、NK细胞及NKT细胞免疫特征，探讨其在HFMD疾病发生发展中的机制及临床检测价值。[方法]应用流式细胞术检测152例HFMD患儿、45例健康对照组幼儿外周血中T淋巴细胞（ CD3＋细胞、CD3＋CD4＋细胞、CD3＋CD8＋细胞）、B淋巴细胞（ CD3－CD19＋细胞）、NK细胞（CD3－CD56＋细胞）、NKT细胞（CD3＋CD56＋细胞）百分率，采用SPSS 13．0统计分析软件，分别对HFMD发病期与健康对照组、HFMD发病期与HFMD恢复期、HFMD重型组与HFMD普通组进行各组淋巴细胞亚群百分率间均数比较，两组间均数的比较方差齐时采用两个独立样本的t检验，方差不齐时用t＇检验，P＜0．05为差异有统计学意义。[结果] HFMD发病期CD3＋细胞、CD3＋CD4＋细胞、CD3＋CD8＋细胞、NK细胞百分率及CD4＋／CD8＋比值低于健康对照组；NKT细胞、B细胞百分率HFMD发病期高于健康对照组，各组间均数的比较，差异均有统计学意义（P＜0．05）。 HFMD发病期CD3＋细胞、CD3＋CD4＋细胞、CD3＋CD8＋细胞、NKT细胞百分率及CD4＋／CD8＋比值低于HFMD恢复期；B细胞、NK细胞百分率HFMD发病期高于HFMD恢复期，各组间均数的比较，差异均有统计学意义（P＜0．05）。 HFMD重型组CD3＋细胞、CD3＋CD4＋细胞、CD3＋CD8＋细胞、NKT细胞百分率及CD4＋／CD8＋比值低于HFMD普通组；B细胞、NK细胞百分率HFMD重型组高于HFMD普通组，除NKT细胞外，其余各组间均数的比较，差异均有统计学意义（P＜0．05）。[结论] HFMD患儿淋巴细胞亚群免疫功能紊乱。 HFMD发病期淋巴细胞亚群中CD3＋细胞、CD3＋CD4＋细胞百分率降低，B细胞百分率增高；HFMD恢复期NKT细胞百分率增高。淋巴细胞亚群免疫特征检测可作为HFMD患儿疾病转归分析指标。
[목적]총결수족구병（ HFMD）환인외주혈T림파세포、B림파세포、NK세포급NKT세포면역특정，탐토기재HFMD질병발생발전중적궤제급림상검측개치。[방법]응용류식세포술검측152례HFMD환인、45례건강대조조유인외주혈중T림파세포（ CD3＋세포、CD3＋CD4＋세포、CD3＋CD8＋세포）、B림파세포（ CD3－CD19＋세포）、NK세포（CD3－CD56＋세포）、NKT세포（CD3＋CD56＋세포）백분솔，채용SPSS 13．0통계분석연건，분별대HFMD발병기여건강대조조、HFMD발병기여HFMD회복기、HFMD중형조여HFMD보통조진행각조림파세포아군백분솔간균수비교，량조간균수적비교방차제시채용량개독립양본적t검험，방차불제시용t＇검험，P＜0．05위차이유통계학의의。[결과] HFMD발병기CD3＋세포、CD3＋CD4＋세포、CD3＋CD8＋세포、NK세포백분솔급CD4＋／CD8＋비치저우건강대조조；NKT세포、B세포백분솔HFMD발병기고우건강대조조，각조간균수적비교，차이균유통계학의의（P＜0．05）。 HFMD발병기CD3＋세포、CD3＋CD4＋세포、CD3＋CD8＋세포、NKT세포백분솔급CD4＋／CD8＋비치저우HFMD회복기；B세포、NK세포백분솔HFMD발병기고우HFMD회복기，각조간균수적비교，차이균유통계학의의（P＜0．05）。 HFMD중형조CD3＋세포、CD3＋CD4＋세포、CD3＋CD8＋세포、NKT세포백분솔급CD4＋／CD8＋비치저우HFMD보통조；B세포、NK세포백분솔HFMD중형조고우HFMD보통조，제NKT세포외，기여각조간균수적비교，차이균유통계학의의（P＜0．05）。[결론] HFMD환인림파세포아군면역공능문란。 HFMD발병기림파세포아군중CD3＋세포、CD3＋CD4＋세포백분솔강저，B세포백분솔증고；HFMD회복기NKT세포백분솔증고。림파세포아군면역특정검측가작위HFMD환인질병전귀분석지표。
Objective] To summarize the immune characteristics in hand foot and mouth disease (HFMD) children with regards to peripheral blood T lymphocyte , B lymphocyte, NK cell and NKT cell, and to discuss its mechanism in the development of HFMD and their detection and clinical value . [ Methods] By means of flow cytometry , 152 children with HFMD and 45 healthy children were detected for the percentage of T lymphocyte , B lymphocyte, NK cell, NKT cell in peripheral blood . [ Results] The percentage of CD3 +,CD3 +CD4 +, CD3 +CD8 +, NK cells and ratio of CD4 +/CD8 +of HFMD acute phase group were lower than those in healthy group , while NKT cells, B cells were higher.The percentage of CD3 +, CD3 +CD4 +, CD3 +CD8 +, NKT cells and CD4 +/CD8 +ratio of HFMD acute phase were lower than those of recovery phase , while B cells and NK cells were higher .The percentage of CD 3 +cells, CD3 +CD4 +cells, CD3 +CD8 +cells, NKT cells and the ratio of CD 4 +/CD8 +in severe HFMD group were lower than those in mild group , while B cells and NK cells were higher . [ Conclusion] The immune function of children with HFMD proved disordered;CD3 +cells, CD3 +CD4 +cells and B cells in peripheral lympho-cytes were increased during the acute phase of HFMD;NKT cells increased during recovery phase of HFMD.We conclude that lymphocyte subsets detection can be used as an index of prognosis of HFMD in children .