CAJ | 학술논문

目的：检测膝骨关节炎（osteoarthritis，OA）患者血清及关节液中白细胞介素-17（in-terleukin-17）的表达并评估其与 OA 严重程度的相关性。方法收集100例原发性膝 OA 患者血清及关节液标本，并以50例健康志愿者血清标本作为对照。OA 严重程度及分级分别采用 Lequesne指数和 K-L 分级系统。酶联免疫吸附法分别检测血清及关节液中 IL-17浓度，并分析其与 OA 严重程度的相关性。结果OA 组患者血清中 IL-17浓度[（5．292±1．470）pg／ml]明显高于对照组[（4．173±0．640）pg／ml]，差异有统计学意义（P ＜0．05）。K-L4级或3级 OA 患者关节液 IL-17的浓度明显高于2级 OA 患者，差异有统计学意义（P ＜0．05）。4级 OA 患者关节液 IL-17的浓度[（6．161±1．120）pg／ml]明显高于3级 OA 患者[（4．474±0．816）pg／ml]，差异有统计学意义（P ＜0．05）。关节液 IL-17浓度与 Lequesne 指数呈正相关（r ＝0．6232，P ＜0．05）；血清 IL-17浓度与 Lequesne 指数之间无明显相关。结论IL-17在 OA 患者血清及关节液中的表达明显升高，关节液中 IL-17的水平与 OA 严重程度之间明显相关，提示 IL-17的失衡表达在 OA 的发病机制中具有一定作用。
목적：검측슬골관절염（osteoarthritis，OA）환자혈청급관절액중백세포개소-17（in-terleukin-17）적표체병평고기여 OA 엄중정도적상관성。방법수집100례원발성슬 OA 환자혈청급관절액표본，병이50례건강지원자혈청표본작위대조。OA 엄중정도급분급분별채용 Lequesne지수화 K-L 분급계통。매련면역흡부법분별검측혈청급관절액중 IL-17농도，병분석기여 OA 엄중정도적상관성。결과OA 조환자혈청중 IL-17농도[（5．292±1．470）pg／ml]명현고우대조조[（4．173±0．640）pg／ml]，차이유통계학의의（P ＜0．05）。K-L4급혹3급 OA 환자관절액 IL-17적농도명현고우2급 OA 환자，차이유통계학의의（P ＜0．05）。4급 OA 환자관절액 IL-17적농도[（6．161±1．120）pg／ml]명현고우3급 OA 환자[（4．474±0．816）pg／ml]，차이유통계학의의（P ＜0．05）。관절액 IL-17농도여 Lequesne 지수정정상관（r ＝0．6232，P ＜0．05）；혈청 IL-17농도여 Lequesne 지수지간무명현상관。결론IL-17재 OA 환자혈청급관절액중적표체명현승고，관절액중 IL-17적수평여 OA 엄중정도지간명현상관，제시 IL-17적실형표체재 OA 적발병궤제중구유일정작용。
Objective To determine the interleukin(IL)-17 concentrations in serum and synovi-al fluid from patients with knee osteoarthritis(OA),and evaluate their correlation with disease severity. Methods Serum and synovial fluid were collected from 100 patients with primary knee OA;age-and sex-matched 50 healthy subjects provided serum samples as controls.OA severity and grade were assessed u-sing the Lequesne index and Kellgren and Lawrence(K-L)grading system,respectively.The expression of IL-17 was quantified by enzyme-linked immunosorbent assay.Results Serum IL-17 concentrations were significantly higher in patients than in controls(P <0.05).In the patient group,patients with K-L grade 3 and 4 had higher synovial IL-17 levels than those with K-L grade 2(P <0.05).At the same time,com-pared with K-L grade 3,there was a higher IL-17 level in K-L grade 4 patients(P <0.05).Synovial fluid IL-17 concentration was positively correlated with Lequesne index(r =0.6232,P <0.05).There was no correlation between serum IL-17 concentrations and Lequesne index,however.Conclusion The expres-sion of IL-17 was significantly increased in serum and synovial fluid of knee from patients with knee OA. There was a positive correlation between synovial fluid IL-17 concentrations and OA severity.It suggests that the imbalance of IL-17 expression plays a certain role in the pathogenesis of OA.