内蒙古中医药
內矇古中醫藥
내몽고중의약
INNER MONGOL JOURNAL OF TRADITIONAL CHINESE MEDICINE
2015年
4期
112-113
,共2页
清热解毒合剂%黄芩苷%薄层层析%高效液相色谱
清熱解毒閤劑%黃芩苷%薄層層析%高效液相色譜
청열해독합제%황금감%박층층석%고효액상색보
heat clearing and detoxicating mixture%baicalin%TLC%HPLC
目的:建立清热解毒合剂的质量控制标准。方法:采用薄层色谱法对制剂中金银花和栀子进行鉴别;采用高效液相色谱法测定制剂中黄芩苷含量。结果:薄层色谱斑点清晰,分离度较好,专属性强。高效液相色谱法测定结果,黄芩苷在0.186~1.861μg线性关系良好。(r=0.9998),平均加样回收率为100.54%,RSD0.41%。结论:该方法快速、灵敏、准确,可有效控制清热解毒合剂的质量。
目的:建立清熱解毒閤劑的質量控製標準。方法:採用薄層色譜法對製劑中金銀花和梔子進行鑒彆;採用高效液相色譜法測定製劑中黃芩苷含量。結果:薄層色譜斑點清晰,分離度較好,專屬性彊。高效液相色譜法測定結果,黃芩苷在0.186~1.861μg線性關繫良好。(r=0.9998),平均加樣迴收率為100.54%,RSD0.41%。結論:該方法快速、靈敏、準確,可有效控製清熱解毒閤劑的質量。
목적:건립청열해독합제적질량공제표준。방법:채용박층색보법대제제중금은화화치자진행감별;채용고효액상색보법측정제제중황금감함량。결과:박층색보반점청석,분리도교호,전속성강。고효액상색보법측정결과,황금감재0.186~1.861μg선성관계량호。(r=0.9998),평균가양회수솔위100.54%,RSD0.41%。결론:해방법쾌속、령민、준학,가유효공제청열해독합제적질량。
Objective: to establish the quality control standards of heat clearing and detoxicating mixture. Methods: Honeysuckle and Gardenia were identified by TLC.The content of baicalin was determined by RP-HPLC.Result: The spots is the TLC were fairly clear, good separating degree, strong specificity. The results of determination by HPLC,a good linear relationship within a range of 0.186~1.861 μ g. (r=0.9998),The average rate of recovery was 100.54%, RSD 0.41%. Conclusion: The method is a simple,rapid and accurate, analysis assay ,which can be used fora quality control of heat clearing and detoxicating mixture.