CAJ | 학술논문

目的：探讨新诊断2型糖尿病患者糖负荷后胰升糖素样肽-1（GLP-1）水平的变化。方法对30例新诊断2型糖尿病患者（糖尿病组）和10例健康志愿者（对照组）行口服葡萄糖耐量试验（OGTT）。分别于空腹，口服葡萄糖后30、60、120和180 min 各采集静脉血10 mL，测定血糖（葡萄糖氧化酶法）、血浆胰岛素（电化学发光免疫法）和GLP-1（7～36，酶联免疫吸附法）。观察 OGTT 中各时间点血糖、血浆胰岛素和 GLP-1（7～36）水平的变化。结果糖尿病组患者在 OGTT 过程中各时间点（空腹，餐后30、60、120和180 min）的血糖水平均高于对照组（均 P ＜0．05）。糖尿病组空腹及口服葡萄糖后120 min 胰岛素水平与对照组比较差异无统计学意义（P ＞0．05）；糖尿病组口服葡萄糖后胰岛素水平于60 min 达峰值，对照组胰岛素水平于30 min 达峰值，糖尿病组胰岛素峰值水平均低于对照组（P ＜0．05）；糖尿病组口服葡萄糖后180 min 胰岛素水平高于对照组、胰岛素曲线下面积则低于对照组（均P ＜0．05）。糖尿病组空腹和口服葡萄糖后各时间点的 GLP-1水平及曲线下面积与对照组比较差异无统计学意义（P ＞0．05）。结论新诊断2型糖尿病患者口服葡萄糖后 GLP-1（7～36）水平无异常。
목적：탐토신진단2형당뇨병환자당부하후이승당소양태-1（GLP-1）수평적변화。방법대30례신진단2형당뇨병환자（당뇨병조）화10례건강지원자（대조조）행구복포도당내량시험（OGTT）。분별우공복，구복포도당후30、60、120화180 min 각채집정맥혈10 mL，측정혈당（포도당양화매법）、혈장이도소（전화학발광면역법）화GLP-1（7～36，매련면역흡부법）。관찰 OGTT 중각시간점혈당、혈장이도소화 GLP-1（7～36）수평적변화。결과당뇨병조환자재 OGTT 과정중각시간점（공복，찬후30、60、120화180 min）적혈당수평균고우대조조（균 P ＜0．05）。당뇨병조공복급구복포도당후120 min 이도소수평여대조조비교차이무통계학의의（P ＞0．05）；당뇨병조구복포도당후이도소수평우60 min 체봉치，대조조이도소수평우30 min 체봉치，당뇨병조이도소봉치수평균저우대조조（P ＜0．05）；당뇨병조구복포도당후180 min 이도소수평고우대조조、이도소곡선하면적칙저우대조조（균P ＜0．05）。당뇨병조공복화구복포도당후각시간점적 GLP-1수평급곡선하면적여대조조비교차이무통계학의의（P ＞0．05）。결론신진단2형당뇨병환자구복포도당후 GLP-1（7～36）수평무이상。
ABSTRACT:Objective To investigate the changes in glucagon-like peptide-1 (GLP-1)secretion after glucose load in patients with newly diagnosed type 2 diabetes.Methods Oral glucose toler-ance test (OGTT)was performed in 30 patients with newly diagnosed type 2 diabetes (diabetes group)and 10 healthy volunteers (control group).Venous blood samples (10 mL)were collected in the fasting state and at 30,60,120 and 180 minutes after oral glucose uptake.Plasma levels of glucose,insulin and GLP-1 (7-36)were measured using glucose oxidase method,electrochemilu-minescence immunoassay and enzyme linked immunosorbent assay,respectively.Results Com-pared with control group,plasma glucose levels significantly increased in diabetes group at all time points (P <0.05).There were no significant differences in insulin levels between the two groups in the fasting state and at 120 minutes after oral glucose uptake (P >0.05).Plasma insulin levels reached the peak at 60 minutes after oral glucose uptake in diabetes group and at 30 minutes after oral glucose uptake in control group.The peak insulin levels in diabetes group were signifi-cantly lower than those in control group (P <0.05).In addition,compared with control group,in-sulin levels increased at 180 minutes after oral glucose uptake and area under the curve (AUC)of insulin decreased in diabetes group (P <0.05).No significant differences in levels and AUC of GLP-1 between the two groups at all time points (P >0.05).Conclusion No changes in GLP-1 (7-36)levels occur after oral glucose load in patients with newly diagnosed type 2 diabetes.